Fluorescence quantification PCR (Polymerase Chain Reaction) primer, probe and kit for detecting dog leptospira nucleic acid

A Leptospira, fluorescence quantitative technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of large gene sequence differences, large gene mobility, and low number of Leptospira
CN103205502AInactive Publication Date: 2013-07-17YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2013-07-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a fluorescence quantification PCR (Polymerase Chain Reaction) primer, a fluorescence quantification PCR probe and a fluorescence quantification PCR kit for detecting dog leptospira nucleic acid. The primer and the probe are shown as SEQ ID NO. (Sequence Identification Number) 1-4. The kit comprises 22 microliters of 5xFRET (Fluorescence Resonance Energy Transfer)-qPCRStock and 22 microliters of 5xOligo mixture, wherein the Oligo mixture comprises the primer and the probe. With the adoption of the primer and the probe, the pathogenic dog leptospira can be detected quickly, specifically and sensitively.
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Description

technical field

[0001] The invention relates to fluorescent quantitative PCR detection primers, probes and kits for the diagnosis of pathogenic dog Leptospira. This invention sensitively, specifically, and rapidly amplifies pathogenic Leptospira interrogans Canicola Canicola, L. interrogans Icterohemorrhagiae Icterohemorrhagiae, L. interrogans Pomona Pomona, and L. kerschneri Grippotyphosa Grippotyphosa that infect dogs, without amplifying non- Pathogenic Leptospira (L. biflexa) nucleic acid. Background technique

[0002] Leptospira can cause a variety of important zoonotic diseases in animals and humans. The main existing laboratory diagnostic techniques for Leptospira infection include: 1) Clinical diagnosis. The clinical symptoms caused by Leptospira are not specific, and the value in clinical diagnosis is limited; 2) Isolation of pathogens: it takes a long time to develop, and requires specially trained professionals; 3) Serological tests to detect anti-Leptospira Ant...

Claims

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