A kind of magnetic nano gene carrier system and its preparation and application

A gene carrier and magnetic nanotechnology, applied in the direction of gene therapy, the use of carriers to introduce foreign genetic material, and other methods of inserting foreign genetic materials, can solve the problem of low transfection efficiency of magnetic gene carriers and achieve improved gene transfection efficiency , The preparation method is simple, the effect of reducing particle size and surface charge

Active Publication Date: 2016-01-20
SICHUAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to address the shortcomings of magnetic gene carriers, especially for the transfection efficiency of existing magnetic gene carriers under serum conditions or in vivo applications Lower problem, provides a magnetic nano-gene carrier system and its preparation and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of magnetic nano gene carrier system and its preparation and application
  • A kind of magnetic nano gene carrier system and its preparation and application
  • A kind of magnetic nano gene carrier system and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0031] The gene was dissolved in sterile water, and the cationic carrier and negatively charged magnetic nanoparticles were dissolved in sterile buffer solution; according to the mass ratio of the cationic carrier to the gene was 0.3:1, the cationic carrier was added to the gene solution, at room temperature Leave it for 8 minutes to obtain the solution binary gene complex; then add negatively charged magnetic nanoparticles into the binary gene complex according to the ratio of iron element to gene mass ratio of 0.2:1 (the average of the magnetic nanoparticles The particle size is 20nm, and the surface potential is

[0032] -25mV), and placed at room temperature for 6 minutes to obtain a three-component magnetic nano-gene carrier system.

Embodiment 2

[0034] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0035] The gene was dissolved in sterile water, and the cationic carrier and negatively charged magnetic nanoparticles were dissolved in sterile buffer respectively; according to the mass ratio of the cationic carrier to the gene was 45:1, the gene solution was added to the cationic carrier, at room temperature Leave it for 30 minutes to obtain the solution binary gene complex; then add negatively charged magnetic nanoparticles into the binary gene complex (the average The particle size is 50nm, and the surface potential is

[0036] -48mV), and placed at room temperature for 25 minutes to obtain a ternary magnetic nano-gene carrier system.

Embodiment 3

[0038] A magnetic nanometer gene carrier system is composed of negatively charged magnetic nanoparticles (MNP), a cationic carrier and a gene. The gene carrier system is prepared by the following method:

[0039] Dissolve the gene in sterile water, and dissolve the cationic carrier and negatively charged magnetic nanoparticles in sterile buffer respectively (the average particle size of the magnetic nanoparticle is 12nm, and the surface potential is -18mV); The mass ratio of the iron element to the gene is 12:1, and the cationic carrier is added to the negatively charged magnetic nanoparticles, and left at room temperature for 18 minutes to obtain a binary complex; Add the gene solution into the binary complex, and place it at room temperature for 12 minutes to obtain a ternary magnetic nano-gene carrier system.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
surface potentialaaaaaaaaaa
particle sizeaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a magnetic nanometer gene carrier system and its preparation and application. The gene carrier system is composed of negatively charged magnetic nanoparticles, cationic carriers and genes; the three components interact according to a certain proportion and composite sequence through electrostatic interaction A magnetic nanometer gene carrier system is prepared. The magnetic nano-gene carrier system of the invention has a simple preparation method and has the physical and chemical properties of small particle size and suitable surface charge. Compared with the non-magnetic nanocomposite, under the action of an external magnetic field, its transfection efficiency to cells is significantly improved. At the same time, its cytotoxicity is also significantly reduced.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a magnetic nano-gene carrier system, a preparation method and an application. Background technique [0002] In recent years, gene therapy has become one of the most active fields in the research of genetic diseases and tumor treatment. The study of gene carrier is the core of gene therapy research. Due to the advantages of safety and low toxicity of non-viral gene vectors, it has become a hotspot in the research of gene vectors. Most non-viral vectors show high gene transfection efficiency in vitro, but low transfection efficiency in target tissues in vivo. Therefore, in the process of gene therapy, the targeting of gene carrier is one of the bottlenecks of gene therapy. [0003] The magnetic targeting gene delivery system has the advantages of high gene expression efficiency in vitro and good targeting in vivo, making it a very promising vector system. At present, the mos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87C12N15/85A61K48/00
Inventor 顾忠伟聂宇谢丽何一燕姜倩岳冬江雯刘克霞
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap