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Ginsenoside conversion method by use of ginseng endophytic Paenibacillus polymyxa

A technology of Paenoid polymyxa and ginsenosides, which is applied in the biological field and can solve the problems of low content of ginsenoside monomers

Active Publication Date: 2015-04-01
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] In view of the low content of ginsenoside monomers in ginseng, the purpose of the present invention is to provide a method for transforming ginsenosides by endogenous Paenibacillus polymyxa in ginseng, so as to produce rare saponins or increase their yield, and then improve the medicinal value of ginseng and bioavailability

Method used

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Examples

Experimental program
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Effect test

example 1

[0004] Example 1 A method for transforming ginsenosides from ginseng endogenous Paenibacillus polymyxa

[0005] The invention relates to the field of biotechnology, and relates to a method for transforming ginsenosides by endophytic Paenibacillus polymyxa in ginseng. The endophytic Paenibacillus polymyxa of the present invention is preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC 7250. The ginseng endophytic Paenibacillus polymyxa of the invention has relatively high transformation activity. The method increases the content of ginsenoside monomers Rg1, Re, Rf, Rg2, Rb1, Rb2, Rb3 and Rd. The bacterial strain can directly use ginseng powder, which is a raw material for transforming saponins, as a culture medium, and the method is simple and low in cost, and is favorable for large-scale industrial production. The specific plan is as follows:

[0006] 1. A ginseng endophytic ...

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Abstract

The invention relates to the field of biotechnology and specifically discloses a ginsenoside conversion method by the use of ginseng endophytic Paenibacillus polymyxa. The endophytic Paenibacillus polymyxa was preserved in the China General Microbiological Culture Collection Center on February, 5th, 2013. The address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The collection number is CGMCC No. 7250. The ginseng endophytic Paenibacillus polymyxa has high conversion activity. By the adoption of the method, contents of ginsenoside monomer Rg1, Re, Rf, Rg2, Rb1, Rb2, Rb3 and Rd are raised or new compounds are induced. According to the strain, a raw material ginseng powder can be directly used as a medium for conversion of saponins. The method is simple, requires low cost, and is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a ginseng endogenous Paenibacillus polymyxa ( Paenibacillus polymyxa ) method for transforming ginsenosides. Background technique [0002] Ginsenosides are the medicinal ingredients of ginseng, but the content of ginseng is relatively low, especially the content of rare ginsenosides. In addition, after oral administration, ginsenosides can hardly be decomposed by human gastrointestinal digestive enzymes, but can only be absorbed and utilized by the human body after being metabolized by the unique microorganisms in the intestinal tract, so as to play a pharmacological role. The possible transformation of panaxadiol saponins is mainly through the enzymes produced by microorganisms to hydrolyze the β-(1-6)-glucosidic bond on C20 and the β-(1-2)-glucosidic bond on C3, and then generate Rd, Rd Further hydrolysis generates F2 or Rg3, F2 is then hydrolyzed into C-K, Rg3 can be converted int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/01
Inventor 郜玉钢张连学臧埔王玉方赵岩杨鹤何忠梅祝鸿燕李学赵丽郭冲
Owner JILIN AGRICULTURAL UNIV