Preprocessing method capable of effectively improving efficiency of generating fermentable sugar by bamboo cellulose enzyme hydrolysis

A cellulase, pretreatment technology, used in fermentation and other directions

Inactive Publication Date: 2013-08-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to overcome the defect that the existing pretreatment method is applied to bamboo, and to provide a kind of pretreatment method that can effectively improve the efficiency of fermentable sugar produced by enzymatic hydrolysis of bamboo cellulose

Method used

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  • Preprocessing method capable of effectively improving efficiency of generating fermentable sugar by bamboo cellulose enzyme hydrolysis
  • Preprocessing method capable of effectively improving efficiency of generating fermentable sugar by bamboo cellulose enzyme hydrolysis
  • Preprocessing method capable of effectively improving efficiency of generating fermentable sugar by bamboo cellulose enzyme hydrolysis

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Embodiment 1

[0050] (1) Select 2 to 3-year-old bamboo, use a chipper to obtain bamboo chips with a three-dimensional size of 30×15×5mm, and use them as raw materials;

[0051] (2) High-boiling alcohol pretreatment, the technical conditions are: liquid-solid ratio of 6 (ratio of high-boiling alcohol pretreatment liquid volume to bamboo chip raw material mass, ml:g), high-boiling alcohol 1,4-butanediol The volume ratio in the pretreatment liquid (composed of alcohol and water) is 10% (% refers to volume percentage, ml: ml), and 180°C is selected in the pretreatment temperature range of 170~210°C for the experiment. The experimental results are shown in Table 1 As shown, the pretreatment time is 25min, and the concentration value of sulfuric acid in the pretreatment liquid as a catalyst is shown in Table 1. After the pretreatment is completed, the small tank in the oil bath reactor is immediately taken out, cooled with tap water, and then placed on a double-layer Filter on a Buchner funnel of...

Embodiment 2

[0057] (1) Select 2 to 3-year-old bamboo, use a chipper to obtain bamboo chips with a three-dimensional size of 30×15×5mm, and use them as raw materials;

[0058] (2) High-boiling alcohol pretreatment, the technical conditions are: the liquid-solid ratio is 6 (the ratio of high-boiling alcohol pretreatment liquid volume and bamboo chip raw material quality, ml: g), and the high-boiling alcohol 1,4 - The volume ratio of butanediol in the pretreatment solution (composed of alcohol and water) is 0~100% (% refers to the volume percentage, ml: ml) and select 0%, 10%, 20%, 35%, 40% in the range , 50%, 65%, 80% and 100% for experiments, see the experimental results figure 1 As shown, the pretreatment temperature is 190 ° C, and the pretreatment time is 45 minutes. After the pretreatment is completed, the small tank in the oil bath reactor is immediately taken out, cooled with tap water, and then filtered on a Buchner funnel with double-layer filter paper. solid and liquid separation...

Embodiment 3

[0063] (1) Select 2 to 3-year-old bamboo, use a chipper to obtain bamboo chips with a three-dimensional size of 30×15×5mm, and use them as raw materials;

[0064] (2) High-boiling alcohol pretreatment, the technical conditions are: liquid-solid ratio of 6 (ratio of high-boiling alcohol pretreatment liquid volume to bamboo chip raw material mass, ml:g), high-boiling alcohol 1,4-butanediol The volume ratio in the pretreatment solution (composed of alcohol and water) is 40% (% refers to the volume percentage, ml: ml), and the pretreatment temperature range is 170~210°C, and 190°C is selected for the experiment, and the pretreatment time is 45min. After the treatment is completed, the small tank in the oil bath reactor is immediately taken out, cooled with tap water, and then filtered on a Buchner funnel with a double layer of filter paper to separate the solid from the liquid;

[0065] (3) Dilute sulfuric acid, dilute alkali, acid sulfite and ethanol / water pretreatment, the techn...

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Abstract

The invention discloses a preprocessing method capable of effectively improving the efficiency of generating fermentable sugars by bamboo cellulose enzyme hydrolysis. The preprocessing method comprises the following steps of: preparing materials; preprocessing acid catalyzed high-boiling alcohol; washing solid substrates obtained by preprocessing; milling the solid substrates by a millstone; and performing enzyme hydrolysis. By preprocessing the acid catalyzed high-boiling alcohol in the preprocessing method disclosed by the invention, hemicellulose and lignin can be synchronously and efficiently removed from bamboo fiber raw materials, and additionally, extractives and waxiness on the surfaces of the extractives can be effectively removed from the bamboo outer skin of the outer wall of bamboos. Compared with the existing acidic chemical preprocessing method, the recovery rate of fermentable glucose obtained by enzyme hydrolysis of cellulose in the bamboos after the high-boiling alcohol preprocessing is increased by 36 to 50%, and compared with the preprocessing method by using a low-boiling organic solvent, the recovery rate is increased by 10 to 15%. By using the preprocessing method in which a high-boiling alcohol/hydrosolvent system with acid catalysis and low alcohol content is utilized and the bamboos are preprocessed before enzyme hydrolysis within short time, the conversion efficiency of the cellulose in the bamboos to the fermentable sugars through enzyme hydrolysis can be improved, and the defect that the environmental pollution is caused by waste liquor can be overcome.

Description

technical field [0001] The invention relates to a pretreatment method of bamboo, in particular to a pretreatment method which can effectively improve the efficiency of fermentable sugar produced by enzymatic hydrolysis of bamboo cellulose. Background technique [0002] Due to the limited reserves of fossil energy and environmental pollution caused by excessive use, most countries in the world have now turned their attention to the development of natural renewable clean energy, such as cellulosic fuel ethanol. In view of the fact that countries are gradually restricting the production of bioethanol from food as raw materials, the transformation of lignocellulosic raw materials with large yields, wide distribution and sustainable use into bioethanol has become the most important research focus in countries all over the world. However, my country's forest resources are relatively scarce, and non-timber resources are relatively abundant. For example, there are more than 40 gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 罗小林黄六莲陈礼辉曹石林张亮华
Owner FUJIAN AGRI & FORESTRY UNIV
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