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Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of corncobs and sesame seed cakes

A technology of probiotic preparation and sesame meal, which is applied in animal feed, animal feed, application, etc., can solve the problem of difficult digestion of monogastric animals, and achieve the effect of low-cost, high-efficiency biotransformation and strong enzyme production capacity.

Inactive Publication Date: 2014-07-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of corncobs, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of corncobs, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, and ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 43.22IU / g; total cellulase activity reaches 3.98IU / g; beta-glucosidase activity reaches 3.98IU / g; reached 11.22IU / g; endoglucanase activity reached 35.45IU / g; xylan...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of corncobs, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of corncobs, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, and ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 42.78IU / g; total cellulase activity reaches 3.86IU / g; beta-glucosidase activity reaches 3.86IU / g; reached 11.15IU / g; endoglucanase activity reached 35.12IU / g...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of corncobs, 5kg of sesame cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 40°C After culturing for 36 hours, a starter culture was formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of corncobs, 300kg of sesame cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and ferment at 35°C for 5 Day, get the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 45.56IU / g; total cellulase activity reaches 4.08IU / g; beta-glucosidase activity reaches 45.56IU / g; reached 10.43IU / g; endoglucanase activity reached 33.59IU / g; xylanase activ...

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Abstract

The invention discloses a method for preparing a complex enzyme-probiotic preparation for feeds through biotransformation of corncobs and sesame seed cakes. The method comprises the steps of fully mixing Puer ripe tea subjected to pile fermentation, lentinus edobes sticks, agrocybe cylindracea sticks, first corncobs, first sesame seed cakes, first wheat bran and first apple pomace with first water so as to form a leaven after acclimation and culture; and fully mixing the leaven, second corncobs, second sesame seed cakes, second wheat bran and second apple pomace with second water so as to obtain the complex enzyme-probiotic preparation for feeds after fermentation. The complex enzyme-probiotic preparation for feeds, with better performance indexes, is prepared by fermenting corncobs and sesame seed cakes with food-grade safe floras with stable micro-ecology, thus achieving low-cost and efficient biotransformation of corncobs and sesame seed cakes. Meanwhile, the method has the advantages of easiness in microorganism growth, high enzyme activity and extensive fermentation processes, and easily achieves large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for preparing a compound enzyme and probiotic preparation for feed by using microecologically stable food-grade safe flora to ferment corn cobs and sesame cakes. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖纪晓燚伍星主吴渊于骅陈功诸文颖杨璐杜姗姗
Owner ZHEJIANG UNIV
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