Pool healthy culture micro-ecologic water purifier

A water purifier and micro-ecological technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc., can solve the problem of low survival rate of effective viable bacteria, unstable product quality, use Unsatisfactory effect and other problems, to achieve significant water purification effect, stable water purification effect, and easy large-scale production

Inactive Publication Date: 2014-02-26
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kind of micro-ecological water purifier for healthy pond culture, which has the advantages of simple production process, low cost and energy consumption, high effective viable bacteria count, and long shelf life. Long, adaptable, and easy to mass-produce, it solves the problems of unstable product quality, unsatisfactory use effect, low survival rate of effective viable bacteria and high production cost in the current market of aquatic product microecological preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of seed culture solution: inoculate a ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and shake the shaker for 20 hours at 30°C and 180r / min to make the seed culture solution The cell concentration reached 4.1×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture it on a shaker at 35°C and 200r / min for 24h to make the seed culture medium Bacteria concentration reached 3.5×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker at 28°C and 200r / min for 24 hours to make the bacteria in the seed culture medium The concentration reaches 8.3×10 7 cfu / mL; Inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker at 30°C and 220r / min for 36 hours to make the bacteria i...

Embodiment 2

[0027] Preparation of seed culture solution: Inoculate one ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and culture it on a shaker at 35°C and 200r / min for 18 hours to make the seed culture solution The cell concentration reached 2.3×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture on a shaker for 30 hours at 37°C and 220r / min to make the seed culture medium The cell concentration reached 6.2×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker for 30 hours at 26°C and 180r / min to make the bacteria in the seed culture medium The concentration reaches 1.8×10 8 cfu / mL; Inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker for 30 hours at 28°C and 180r / min to make the ...

Embodiment 3

[0032] Preparation of seed culture solution: Inoculate one ring of Bacillus subtilis slant strain cells into the liquid expansion broth nutrient medium, and shake the shaker for 24 hours at 37°C and 220r / min to make the seed culture solution The cell concentration reached 6.0×10 9 cfu / mL; Inoculate a ring of slant strain cells with 1 strain of Bacillus lateralis in the nutrient medium of liquid expansion broth, and culture it on a shaker for 36 hours at 36°C and 180r / min to make the seed culture medium The cell concentration reached 8.9×10 9 cfu / mL; Inoculate a ring of slant strain cells with one strain of Hansenula anomalies in the wort liquid medium, and culture it on a shaker at 27°C and 220r / min for 20h to make the bacteria in the seed culture medium The concentration reaches 5.5×10 7 cfu / mL; inoculate a ring of slant strain cells with one strain of Candida rugosa in the wort liquid medium, and culture it on a shaker at 29°C and 200r / min for 24 hours to make the bacteria...

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PUM

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Abstract

The invention discloses a pool healthy culture micro-ecologic water purifier. According to the pool healthy culture micro-ecologic water purifier, bacillus subtilis, bacillus lateraporus, hansenula anomala and candida versatilis are used as the fermentation strains, the fermentation strains are subjected to solid fermentation under appropriate carbon nitrogen ratio and external condition, and are subsequently dried at low temperature, and a protective agent and a protective agent accelerant are added so as to obtain the pool healthy culture micro-ecologic water purifier which is free of pollution, safe and long in storage life. The pool healthy culture micro-ecologic water purifier can effectively reduce the content of organic pollutants, ammoniacal nitrogen and COD (Chemical Oxygen Demand) in a pool healthy culture water body, and has the capabilities of nitration, denitrification and feed reside degradation, so that the environment of the pool healthy culture water body is improved. By utilizing the product disclosed by the invention, the defects that in the prior art the water purification effect is not stable, the viable count is low, the production cost is low and the like are well overcome; the product is simple in production process and free of wastewater, waste gas and industrial residue emission, and has significance in freshwater healthy culture and sustainable aquatic development.

Description

technical field [0001] The invention belongs to the field of biotechnology in freshwater aquaculture, and in particular relates to a micro-ecological water purifier for healthy pond aquaculture. Background technique [0002] Since the reform and opening up, my country has implemented the fishery development policy of "focusing on farming", and the freshwater aquaculture industry has made great progress. The freshwater pond aquaculture area has reached 37 million mu, the output has exceeded 16 million tons, and the output value has reached nearly 300 billion yuan. Although my country's freshwater pond aquaculture has made great achievements, it also faces a series of problems and development bottlenecks: one-sided pursuit of economic benefits in aquaculture production, over-capacity development of aquaculture waters, blindly expanding the scale of aquaculture, and ignoring the protection of the ecological environment of water areas. In aquaculture activities, farmed fish can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 王红权赵玉蓉兰时乐肖调义
Owner HUNAN AGRICULTURAL UNIV
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