Method for preparing polymeric microsphere in alcohol-water mixed solvent

A technology of mixing solvents and polymers, which is applied in the field of polymer materials and analysis, can solve the problems of specificity decline, achieve the effects of easy operation, mild reaction conditions, and enhanced specific adsorption capacity

Active Publication Date: 2013-09-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF5 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surfactant sodium dodecyl sulfate (SDS) was added in the synthesis process, which can remain on the surface of the polymer micro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing polymeric microsphere in alcohol-water mixed solvent
  • Method for preparing polymeric microsphere in alcohol-water mixed solvent
  • Method for preparing polymeric microsphere in alcohol-water mixed solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In a 25mL round bottom flask, install a condenser, add 2ml of ethanol and 8ml of water to form a mixed solution, add 33.8mg of 4-vinylphenylboronic acid, 221.1mg of methylenebisacrylamide and 5mg of azobis Isobutyronitrile was sonicated for 1 minute to dissolve the added reagent to form a homogeneous solution, after which nitrogen gas was passed for 15 minutes, and a magnetic stirrer was added. The reaction device was placed in an oil bath and heated evenly and slowly, and the temperature rose to 70°C within 30 minutes. The magnetic stirring bar was kept at a speed of 300 rad / min. The reaction conditions were maintained for 24 hours, the reaction was stopped, and cooled to room temperature. Use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, add a mixed solution of ethanol and water to wash three times, and then wash three times with tetrahydrofuran, acetone, and anhydrous ether. Dry for 24 hours to obtain a polymer sample. ...

Embodiment 2

[0027] In a 25mL round bottom flask, install a condenser, add 2ml of ethanol and 8ml of water to form a mixed solution, add 47.35mg of 4-vinylbenzeneboronic acid, 148mg of methylenebisacrylamide, and 45.6μL of divinylbenzene and 5 mg of azobisisobutyronitrile, ultrasonication for 1 min to dissolve the added reagents to form a uniform solution, then pass nitrogen gas for 15 min, and add a magnetic stirrer. The reaction device was placed in an oil bath and heated evenly and slowly, and the temperature rose to 70°C within 30 minutes. The magnetic stirring bar was kept at a speed of 300 rad / min. The reaction conditions were maintained for 24 hours, the reaction was stopped, and cooled to room temperature. Use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, add a mixed solution of ethanol and water to wash three times, and then wash three times with tetrahydrofuran, acetone, and anhydrous ether. Dry for 24 hours to obtain a polymer samp...

Embodiment 3

[0029] In a 25mL round bottom flask, install a condenser, add 2ml of ethanol and 8ml of water to form a mixed solution, add 29.6mg of 4-vinylphenylboronic acid, 185mg of methylenebisacrylamide, 17.1μL of methacrylic acid and 5mg azobisisobutyronitrile, sonicated for 1 min to dissolve the added reagent to form a homogeneous solution, then pass nitrogen gas for 15 min, and add a magnetic stirrer. The reaction device was placed in an oil bath and heated evenly and slowly, and the temperature rose to 70°C within 30 minutes. The magnetic stirring bar was kept at a speed of 300 rad / min. The reaction conditions were maintained for 24 hours, the reaction was stopped, and cooled to room temperature. Use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, add a mixed solution of ethanol and water to wash three times, and then wash three times with tetrahydrofuran, acetone, and anhydrous ether. Dry for 24 hours to obtain a polymer sample. After ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical fields of a polymer material and analysis and relates to a phenylboronic acid polymeric microsphere, and a preparation method and application thereof on the aspect of gathering glycoprotein. The phenylboronic acid polymeric microsphere containing vinyl is prepared in a novel mixed solvent system of water and ethanol. The method comprises the following steps of: dispersing a monomer, a crosslinking agent and an initiator into a mixed solution of water and ethanol; reacting for 8-24 hours under the condition of 60-70 DEG C, so as to collect the product disclosed by the invention in a reaction system. The synthetic method disclosed by the invention is simple and feasible, low in cost, and friendly to environment, and is typical green reaction; and the prepared polymeric microsphere has the characteristics of good monodispersity, large in specific surface area, strong specificity and the like, and can be used for separating or gathering glycoprotein with 1,2-cis diol structures.

Description

technical field [0001] The invention belongs to polymer materials and analysis techniques, and in particular relates to a preparation method of polymer nanospheres containing 4-vinylphenylboronic acid and its application in glycoprotein enrichment. Background technique [0002] With the end of human genome sequencing, proteomics has gradually become the focus of attention, among which the analysis and identification of post-translational modified proteins is the focus of proteomics research. As a very important post-translational modification protein, glycoprotein is involved in cell growth, information transmission between cells, immunity and other processes. However, due to its low content in the organism, it is easily interfered by other non-glycosylated proteins, and it is not easy to ionize in mass spectrometry, so it needs to be enriched before its identification. [0003] The mechanism of boronic acid affinity is that boronic acid groups are combined with 1,2-o-dihyd...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08F230/06C08F220/06C08F222/38C08F212/36B01J20/26B01J20/28B01J20/30C07K1/14
Inventor 张丽华刘键熙曲焱焱杨开广张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap