Trichinella Spiralis larva ES antigen gene vaccine and preparation method

An antigen gene and muscle larvae technology, applied in the field of Trichinella muscle larvae ES antigen gene vaccine and preparation thereof, can solve the problems of complex components of the Trichinella muscle larva ES antigen composition, and achieve easy reversal of elimination and restoration of original activity and structure. simple effect
CN103352047BActive Publication Date: 2015-01-21JILIN AGRICULTURAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN AGRICULTURAL UNIV
Publication Date
2015-01-21

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Abstract

The invention discloses a Trichinella Spiralis larva ES antigen gene vaccine and a preparation method thereof. Trichinella Spiralis larva 43ku and 45ku ES protein genes are respectively inserted to a pVAXI eukaryotic expression vector to obtain recombinant plasmids, and the recombinant plasmids are mixed according to a ratio of 1:1 to prepare a combined recombined DNA vaccine for preventing Trichinella Spiralis infection and improving the worm reduction rate of bodies. After the injection immunization of hindlimb muscles of mice for three weeks, 2000 worms are killed, the worm reduction rate can reach 76.65%, and the quantity of eggs at the tongue tissues of the mice is obviously reduced.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and relates to a trichinella spiralis muscle larva ES antigen gene vaccine and a preparation method thereof. Background technique

[0002] Trichinella spiralis parasitizes humans and various vertebrates, and is a zoonotic parasitic disease that causes trichinellosis. Trichinella can parasitize humans and more than 150 kinds of animals. The main cause of infection is the intake of raw or undercooked meat products with cysts of Trichinella muscle larvae. resulting in the death of the patient. It is estimated that there are approximately 11 million infected people in the world at present. After 1975 in my country, there were also reports of outbreaks of this disease in Jilin, Liaoning, Heilongjiang, Henan, Hubei and other provinces and municipalities. The disease not only seriously endangers human health, but also causes huge economic losses to the pig industry and meat export. The Internation...

Claims

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