A rapid method for evaluating the tolerance of cotton to glyphosate

Abstract

The invention provides a method for rapidly evaluating glyphosate tolerance of cotton. The method comprises: cotton seeds to be tested are planted; in a cotton cotyledon period, solutions with the glyphosate concentration of 0 g a.i. / L, 1.37 g a.i. / L to 2.05 g a.i. / L, 2.73 g a.i. / L to 4.10 g a.i. / L and 8.20 g a.i. / L are respectively sprayed, wherein the group sprayed with the glyphosate concentration of 0 g a.i. / L is used as a control group, and the other groups sprayed with the other concentrations are used as test groups; 3-5 days later after spraying, the content of shikimic acid in cotton cotyledons is measured; and the glyphosate tolerance of the cotton are determined by comparing whether the contents of shikimic acid in the cotton cotyledons of the test groups and the content of shikimic acid in the cotton cotyledons of the control group have significant difference in statistics. The method has the advantages of short consumed time, small occupied space, low cost, high accuracy and good repeatability, is especially suitable for multi-material and large-scale determination, and is easy to popularize and apply in the field of agriculture.

Description

technical field [0001] The present application relates to the technical field of plant protection, and more specifically, relates to a method for rapidly evaluating the tolerance of cotton to glyphosate. Background technique [0002] The harm of weeds in cotton fields is one of the main obstacles to cotton production in my country, causing losses of 14% to 16% all the year round, seriously restricting the high-quality and efficient production of cotton. Glyphosate is an amino acid biosynthesis inhibitor, which mainly inhibits the conversion of shikimic acid to phenylalanine, tyrosine and tryptophan by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in plants. Acid conversion, which interferes with protein synthesis, leads to the accumulation of shikimic acid in plants, and eventually plant death. As a broad-spectrum systemic herbicide, glyphosate plays an important role in the control of weeds in cotton fields. However, because cotton is sensitive to glyphos...

AI Technical Summary

Problems solved by technology

[0004] At present, the evaluation of cotton glyphosate resistance mainly adopts the plant bioassay method, that is, sowing cotton in containers or fields, spraying cotton seedlings with a series of glyphosate solutions after emergence, and according to the death of cotton after application and the impact on yield to evaluate its resistance. This method can accurately measure the resistance level of cotton to glyphosate, but it takes a long time. It takes about 150 days from sowing and harvesting to the determination results. For the determination of multiple materials and large batches, it takes a long time, takes up a lot of space, and has low efficiency

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