Method for marking and identifying target objects

Abstract

The invention provides a method for marking and identifying target objects, which comprises the following steps: carrying out fixation and pretreatment on a cell; respectively marking a first target object and a fourth target object in the cell through two probes with a first fluorescent dye, and respectively marking a second target object, a third target object and a fifth target object in the cell through three probes with a second fluorescent dye, wherein the first target object and the third target object are mutually overlapped, the second target object and the fourth target object are mutually overlapped, the fifth target object and other target objects are not overlapped, the first target object and the fourth target object are different in volume, and the second target object and the third target object are different in volume; carrying out fluorescence imaging on the cell through a first fluorescence imaging system so as to obtain a first fluorescence image and a second fluorescence image; merging the first fluorescence image and the second fluorescence image, calculating the volumes of the target objects, and identifying the multiple target objects according to a situation that whether the fluorescence images are overlapped and the volumes of the target objects.

Description

technical field [0001] The invention relates to a method for marking and identifying a target, in particular to a method for marking and identifying a target using fluorescent light. Background technique [0002] Several studies have shown that chromosomes occupy a distinct region in the nucleus called a chromosomal domain. Chromosomal domains are evolutionarily conserved and their spatial conformation is nonrandom during interphase. Gene (genetic factor) is the material basis of heredity and is a specific nucleotide sequence with genetic information on DNA or RNA molecules. The genes of most organisms are arranged linearly on chromosomes. Chromosomes with high gene density tend to be distributed near the center of the nucleus, while chromosomes with low gene density tend to be distributed in the periphery. In addition, the distribution position of chromosomes in the nucleus also has a certain relationship with the size of the chromosomes themselves. Evidence suggests th...

AI Technical Summary

Problems solved by technology

However, the implementation of the 3D-M-FISH method relies on special image processing software, and the software is expensive

However, the ReFISH method requires the operator to find and label the same cell nucleus twice, which is a very time-consuming and highly technically demanding task for the operator.

It can be seen that the number of labeled targets in these methods is limited by the selected labeling method and the experimental equipment used

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