Analyzing method for bacterial community structures of rhizosphere soil of peanuts in different growth periods
A technology of rhizosphere soil and growth period, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of rhizosphere soil bacterial community structure, unable to fully reveal the peanut rhizosphere soil bacterial structure, etc. To achieve the effect of short cycle and high efficiency
Active Publication Date: 2015-03-18
SHANDONG PEANUT RES INST
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Problems solved by technology
Due to the current situation that most soil bacteria are still unculturable, traditional culture methods cannot fully reveal the bacterial structure of the peanut rhizosphere soil.
Due to the particularity of rhizosphere soil sampling and the high diversity of soil bacteria, effective methods for studying the structure of rhizosphere soil bacterial communities and identifying specific bacterial groups are still lacking.
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[0018] The technical solution of the present invention is further illustrated below through specific examples.
[0019] A method for analyzing the bacterial community structure of the rhizosphere soil in different growth periods of peanuts. Pot experiments are adopted to select four different periods of the seedling stage, flowering stage, berry stage, and harvest stage in the entire growth cycle of peanuts as the basis for studying the bacterial community structure of the rhizosphere soil. point in time. In each period, three peanut plants were randomly selected as repeated samples, and the rhizosphere soil of the three samples were collected respectively. The three soil samples were fully mixed and then quick-frozen with liquid nitrogen, and stored in a -80°C refrigerator for later use. The sampling process requires aseptic operation, and all tools used are sterilized with 75% alcohol. The kit was used to extract soil genomic DNA. The entire extraction process was aseptical...
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The invention discloses an analyzing method for bacterial community structures of rhizosphere soil of peanuts in different growth periods. The analyzing method comprises the following steps of: collecting the rhizosphere soil of the peanuts in different growth periods and respectively extracting DNA (Deoxyribonucleic Acid) of soil genomes; carrying out amplification by a 16SrRNA (16S ribosomal Ribonucleic Acid) gene degenerate primer to obtain a PCR (Polymerase Chain Reaction) product; carrying out homogenization and then purification on the obtained PCR product; then constructing a 16SrRNA gene library; analyzing and comparing obtained library data so as to compressively display the bacterial community structures of the rhizosphere soil of the peanuts in different growth and development periods. According to the analyzing method disclosed by the invention, all common bacterial species and dominant species of the rhizosphere soil of the peanuts in different growth and development periods can be obtained, and the difference of the bacterial community structures of the rhizosphere soil of the peanuts in different growth and development periods is compressively known. The analyzing method disclosed by the invention has the advantages of high efficiency, short period, and capability of comprehensively displaying the specific bacterial species in the rhizosphere soil.
Description
technical field [0001] The invention relates to a method for analyzing the rhizosphere soil bacterial community structure of peanuts in different growth stages, and belongs to the technical field of soil microorganisms and crop cultivation. Background technique [0002] Soil microorganisms participate in 80-90% of the soil cycle process, and soil bacteria are the most abundant and diverse group of soil microorganisms. play a very important role in the formation process. The rhizosphere is a unique circle formed by the interaction between plant roots and soil microorganisms. It is affected by the activities of plant roots and is different from the soil around the roots in terms of physical, chemical and biological properties. The interaction between bacteria and plant roots in this microdomain is more closely, and the community structure of rhizosphere bacteria is closely related to the growth and development of plants. [0003] Peanut is an important oil crop and economic ...
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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 陈明娜杨庆利潘丽娟迟晓元杨珍陈娜王通王冕禹山林
Owner SHANDONG PEANUT RES INST