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Method for evaluating sperm intracorporal conception rate of breeding bull according to extracorporal fertilization rate of breeding bull

A technology for in vitro fertilization and evaluation methods, which is applied in animal husbandry and other fields, and can solve the problems of consuming a lot of manpower, financial resources and time

Active Publication Date: 2013-11-20
内蒙古赛科星繁育生物技术(集团)股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

The reproductive ability of breeding bulls directly affects the quality of frozen semen. It is very important to know the reproductive ability of individual breeding bulls before semen collection, but it takes a lot of manpower, financial resources and time to test the reproductive ability of individual breeding bulls through artificial insemination

Method used

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  • Method for evaluating sperm intracorporal conception rate of breeding bull according to extracorporal fertilization rate of breeding bull
  • Method for evaluating sperm intracorporal conception rate of breeding bull according to extracorporal fertilization rate of breeding bull
  • Method for evaluating sperm intracorporal conception rate of breeding bull according to extracorporal fertilization rate of breeding bull

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Experimental program
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Effect test

Embodiment 1

[0089] Embodiment 1: 1. Wash the fresh bovine ovary within 3 hours collected by the slaughterhouse for 3 times with sterile physiological saline and soak in clean physiological saline; use a 20ml syringe 17 # Extract follicles with a diameter of 2-8mm with a needle, put the collected follicle fluid in a 10cm culture dish, and pick out the cumulus-oocyte complex COCs under a microscope; put the sorted COCs into carbon dioxide at 38.5°C The bovine oocytes equilibrated in the incubator for 1 hour were washed once in egg-picking liquid, and washed twice in bovine oocyte maturation liquid. According to the number of 50 oocytes per well, each well contained 500 μl of bovine oocyte maturation liquid, and the upper cover In a four-well plate with 500 μl paraffin oil, mature culture for 22 hours in a 5% carbon dioxide incubator at 38.5°C;

[0090] 2. Take two 50ml beakers and mark them as A and B respectively: Beaker A prepares BO-A 40ml bovine in vitro semen, beaker B prepares bovine ...

Embodiment 2

[0119] Embodiment 2: the method for conventional evaluation of the conception rate of breeding bulls:

[0120] 1. According to the national standard, the sperm motility after thawing is greater than 30%, the deformity rate is less than 20%, the number of bacterial colonies per milliliter of semen is less than 1000, the acrosome integrity rate is greater than 40%, and the survival time at 37°C is greater than 4 hours. Thin tube frozen semen of 3 breeding bulls;

[0121] 2. It is mainly carried out in large-scale dairy farms. The participating cows randomly select normal estrous cows, and the lactating cows without reproductive system diseases are checked by rectal examination. The estrus of cows is mainly identified by the following methods:

[0122] A. External observation method: mainly judge the estrus status according to the external and mental state of the cow;

[0123] B. Vaginal inspection method: Use a vaginal speculum to observe changes in the vaginal mucous membrane,...

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Abstract

The invention discloses a method for evaluating the sperm intracorporal conception rate of a breeding bull according to the extracorporal fertilization rate of the breeding bull, and belongs to the technical field of animal reproduction. The method includes the steps that COCs is washed by bull oocyte egg picking liquid and bull oocyte mature liquid respectively, placed in the bull oocyte mature liquid for culture, and placed in bull sperm drips capacitated by BO-AB liquid for fertilization; then granular cells are taken off by IVC SOF liquid, then eggs with the granular cells which are taken off are placed in SOF liquid for culture, and the extracorporal fertilization cleavage rate is measured; artificial insemination is conducted, the conception rate of the breeding bull is measured, and the breeding bull is evaluated according to the relevance of the cleavage rate and the conception rate. The method has the advantages that by the utilization of sperm extracorporal fertilization rate of the breeding bull, the practical conception capacity of the breeding bull can be rapidly estimated, the breeding capacity of the breeding bull can be known in time, the problem that a large amount of time, labor and money need to be wasted for detecting the breeding capacity of a breeding bull individual through manual insemination is solved, and resources are saved.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, in particular to a method for evaluating the in vitro fertilization rate of a bull to the in vivo conception rate of a bull's sperm. Background technique [0002] With the widespread application of bovine frozen semen, the quality of individual bulls has also received more and more attention. The reproductive ability of breeding bulls directly affects the quality of frozen semen. It is very important to know the reproductive ability of individual breeding bulls before semen collection, but it takes a lot of manpower, financial resources and time to test the reproductive ability of individual breeding bulls through artificial insemination. Therefore, if the in vitro fertilization rate of the bull's sperm can be used to evaluate the conception rate in the body, it can not only save resources, but also quickly estimate the actual conception ability of the bull, and understand the reprodu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
Inventor 李喜和苏杰韩红梅王春生吴冬生胡树香
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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