Method for evaluating sperm intracorporal conception rate of breeding bull according to extracorporal fertilization rate of breeding bull

A technology of in vitro fertilization and conception rate, which is applied in animal husbandry and other fields, and can solve the problems of consuming a lot of manpower, money and time
CN103392670BActive Publication Date: 2015-06-10内蒙古赛科星繁育生物技术(集团)股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
内蒙古赛科星繁育生物技术(集团)股份有限公司
Publication Date
2015-06-10

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Abstract

The invention discloses a method for evaluating the sperm intracorporal conception rate of a breeding bull according to the extracorporal fertilization rate of the breeding bull, and belongs to the technical field of animal reproduction. The method includes the steps that COCs is washed by bull oocyte egg picking liquid and bull oocyte mature liquid respectively, placed in the bull oocyte mature liquid for culture, and placed in bull sperm drips capacitated by BO-AB liquid for fertilization; then granular cells are taken off by IVC SOF liquid, then eggs with the granular cells which are taken off are placed in SOF liquid for culture, and the extracorporal fertilization cleavage rate is measured; artificial insemination is conducted, the conception rate of the breeding bull is measured, and the breeding bull is evaluated according to the relevance of the cleavage rate and the conception rate. The method has the advantages that by the utilization of sperm extracorporal fertilization rate of the breeding bull, the practical conception capacity of the breeding bull can be rapidly estimated, the breeding capacity of the breeding bull can be known in time, the problem that a large amount of time, labor and money need to be wasted for detecting the breeding capacity of a breeding bull individual through manual insemination is solved, and resources are saved.
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Description

technical field

[0001] The invention belongs to the technical field of animal reproduction, in particular to a method for evaluating the in vitro fertilization rate of a bull to the in vivo conception rate of a bull's sperm. Background technique

[0002] With the widespread application of bovine frozen semen, the quality of individual bulls has also received more and more attention. The reproductive ability of breeding bulls directly affects the quality of frozen semen. It is very important to know the reproductive ability of individual breeding bulls before semen collection, but it takes a lot of manpower, financial resources and time to test the reproductive ability of individual breeding bulls through artificial insemination. Therefore, if the in vitro fertilization rate of the bull's sperm can be used to evaluate the conception rate in the body, it can not only save resources, but also quickly estimate the actual conception ability of the bull, and understand the reprodu...

Claims

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