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Application of gadd45β gene in preparation of anti-bladder cancer drug

A GADD45, 1.GADD45 technology, applied in gene therapy, drug combination, anti-tumor drugs, etc.

Active Publication Date: 2014-10-08
四川丰讯科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The signaling pathway of p38MAPK activated by TGF-β mediated by GADD45β plays an important role in the process of TGF-β-mediated tumor suppression, but the function of this gene in bladder cancer tumor cell apoptosis has not been reported

Method used

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  • Application of gadd45β gene in preparation of anti-bladder cancer drug
  • Application of gadd45β gene in preparation of anti-bladder cancer drug
  • Application of gadd45β gene in preparation of anti-bladder cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: GADD45β gene inhibits bladder cancer T24 cells

[0012] 1. Construction of recombinant expression vector

[0013] 1. Preparation of PCR products

[0014] Using the reverse-transcribed cDNA of T24 cell RNA as a template, the expression primers with restriction sites were designed based on the full-length cDNA sequence of GADD45β gene (GeneID: 4616) for PCR amplification. The reaction conditions were: pre-denaturation at 94°C for 3 min, denaturation at 94°C for 30 s, annealing at 60°C for 40 s, extension at 72°C for 1 min, a total of 35 cycles, and incubation at 72°C for 10 min. The primer sequences are shown in Table 1. The PCR product recovery process was carried out strictly according to the instructions of the Gel Extraction Kit of OMEGA Company.

[0015] Table 1 GADD45β expression primers

[0016]

[0017] 2. Full-length verification of the target gene

[0018] The target gene obtained by PCR was connected to Zero Vector and sent to Beijing Aoke Bio...

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Abstract

The invention provides a use of a gene GADD45 beta in preparation of drugs for treating cancers and especially provides a use of the gene GADD45 beta in preparation of a drug for treating bladder cancer. According to the invention, an over-expression vector of the gene GADD45 beta is constructed and is transferred into tumor cells. A result shows that an mRNA level of the tumor cells with the gene GADD45 beta is improved obviously; roundness of the tumor cells is improved and adherence of the tumor cells is reduced; and tumor cell activity is improved obviously by 85% and tumor cells die finally. The gene GADD45 beta has high tumor cell inhibition activity and provides base data for novel drug target discovery and a tumor-treatment drug design.

Description

technical field [0001] The invention relates to a new application of the GADD45β gene, in particular to the application of the GADD45β gene in the preparation of anti-bladder cancer drugs. Background technique [0002] The GADD45 family is composed of three structurally similar small molecular proteins GADD45α, GADD45β and GADD45γ, which will significantly increase the transcription level when cells are in growth arrest or induced by DNA damage agents, and their proteins bind and activate MTK1 / MEKK4 kinases, thereby Mediates activated p38 / JNK pathway. Increased levels of GADD45 are necessary for cell cycle arrest, allowing DNA damage repair, and play an important role in cell growth and apoptosis. [0003] GADD45β is the main response gene for growth inhibition and apoptosis induced by TGF-β, and its encoded nuclear protein can interact with other proteins such as PCNA, p21, core histones, MEKK4 kinase and Cdk1. The signal transduction pathway of p38MAPK activated by TGF-β...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 张建珍宋丽丽马恩波郑耀武任连生
Owner 四川丰讯科技发展有限公司
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