Co-culture method of photosensory precursor cells and retinal tissue in vitro

Abstract

The invention relates to a co-culture method of photosensory precursor cells and retinal tissue in vitro and aims to build a co-culture system for the photosensory precursor cells and denaturation retinal tissue of retina photoreceptor cells. The method comprises the following steps: a photosensory precursor cell layer of an embryonic eye source is prepared; a nerve cell layer of a denatured retina is prepared; the nerve cell layer of the denatured retina is placed on the photosensory precursor cell layer; an epithelial layer of retinochrome is prepared in the lower chamber of a plug-in type tissue culture dish; the co-culture system of the retinal tissue and cells is built in vitro, and the retinal tissue and cells are cultured in an incubator with 5% CO2 at the temperature of 37 DEG C. By adopting the method provided by the invention, the biological characteristics and cellular structure of host cells and transplanted cells, the synaptic contact of the transplanted cells and the host cells, and the chromatin conformation reconstruction of the transplanted cells and the host cells can be observed and detected directly in vitro, and the traces of survival, proliferation, differentiation and functional reconstruction of photosensory precursor cells of a transplanted embryonic eye source can be tracked.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for co-cultivating photosensitive precursor cells and retinal tissue in vitro. Background technique [0002] Cell culture is to place cells under in vitro conditions for growth and reproduction, and to directly observe the life activities such as the morphology and structure of the cells and the cell growth cycle. Cultured cells live independently in an artificially simulated environment, but this environment is quite different from the real in vivo environment. In order to establish a culture system that is more similar to the in vivo environment, and make the in vitro culture environment as consistent as possible with the in vivo environment, so that cells can communicate with each other and support each other for growth and proliferation, people have created cell co-culture on the basis of cell culture technology. technology. Cell co-cultivation technology is the techno...

AI Technical Summary

Problems solved by technology

[0003] At present, in the basic and clinical transformation research of embryonic stem cells in the treatment of blinding eye diseases, the co-culture of two or more cells is far from meeting the requirements of the experiment

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