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Rapid detection method and detection equipment for pathogenic microorganisms

A technology of pathogenic microorganisms and detection methods, applied in the field of rapid microorganism detection, can solve problems such as high reliability of sample background interference, and achieve the effects of improving rapid detection capability, enhancing electrochemical and piezoelectric sensing signals, and high specificity

Active Publication Date: 2013-11-27
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Aiming at many problems such as large sample background interference and poor reliability of relying on a single sensing technology in the current on-site detection of infectious disease pathogens, the purpose of the present invention is to provide a rapid detection method and detection equipment for on-site pathogenic microorganisms

Method used

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  • Rapid detection method and detection equipment for pathogenic microorganisms
  • Rapid detection method and detection equipment for pathogenic microorganisms

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Embodiment Construction

[0031] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are merely exemplary, and do not constitute further limitations on the scope of the present invention defined by the claims.

[0032] Preparation Examples:

[0033] (1) Co-precipitation method to prepare electrochemically active magnetic nanomaterials. The specific preparation method is: select FeCl 2· 4H 2 O and FeCl 3· 6H 2 O is iron source, get 4.25g FeCl 2· 4H 2 O and 2gFeCl 3· 6H 2 O was dissolved in 25mL of 36.5% HCl solution, and the mixture was added dropwise to 250mL, 1.5M NaOH, while stirring mechanically, and after black precipitate appeared, it was then washed by centrifugation, and the obtained product was dried in an oven at 60°C Stand-by; take 0.25g of the prepared product and disperse it in 10mL of deionized water,...

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Abstract

The invention discloses a rapid pathogenic microorganism detection method based on a multifunctional magnetic nano material and a piezoelectric electrochemical sensor, and mobile detection equipment for carrying out the method. The method has the advantages that the detection is rapid, sensitive and specific, and is applicable to the on-site rapid detection on pathogenic microorganisms.

Description

technical field [0001] The invention relates to a rapid detection method and detection equipment for on-site pathogenic microorganisms, belonging to the technical field of rapid detection of microorganisms. Background technique [0002] Quartz Crystal Microbalance (QCM, Quartz Crystal Microbalance) utilizes the piezoelectric properties of the quartz crystal resonator to convert the surface quality change of the quartz crystal oscillator electrode into the frequency change of the output electrical signal of the quartz crystal oscillating circuit, and then obtain it through a computer and other auxiliary equipment. High-precision data, its measurement accuracy can reach nanogram level. Electrochemical quartz crystal microbalance (EQCM, electrochemical quartz crystal microbalance) utilizes both the high sensitivity of electrochemical detection and the characteristics of QCM that can detect surface quality and damping in real time, and can detect nanogram-level mass changes on t...

Claims

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Application Information

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IPC IPC(8): G01N27/27G01N33/577
Inventor 郝荣章宋宏彬李杨林睿邱少富王勇吴志豪王立贵李鹏戚红卷孙中杰李悦黄留玉
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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