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Application of human mir-148a in the preparation of drugs for improving insulin sensitivity of adipocytes

A technique for insulin sensitivity, adipocytes, applied in the field of genetic engineering

Inactive Publication Date: 2016-03-23
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the relationship between this miRNA and adipocyte insulin sensitivity has not been reported

Method used

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  • Application of human mir-148a in the preparation of drugs for improving insulin sensitivity of adipocytes
  • Application of human mir-148a in the preparation of drugs for improving insulin sensitivity of adipocytes
  • Application of human mir-148a in the preparation of drugs for improving insulin sensitivity of adipocytes

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Experimental program
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Embodiment 1

[0014] 1 Materials and methods

[0015] 1.1 Test materials: Human Preadipocytes-visceral (HPA-v) was purchased from Sciencell, USA. The pGLV-H1-GFP / Puro plasmid required for this experiment was purchased from Shanghai Gemma Company, and the packaging plasmids Pcgvp, Rev, and Vsvg were purchased from Allele Biotech & Pharmaceuticals in the United States; Self-constructed; DNA and RNA extraction kits were purchased from Qiagen, Germany; miRNA reverse transcription kits, miRNA detection kits, and TanMan gene expression mix II were purchased from ABI, USA; TaKaRaLA DNAPolymeraseHot-StartVersionPCR kit was purchased from Takara Company of Japan, adipocyte proprietary medium 7211 was purchased from Sciencell Company of the United States, restriction endonuclease and T4 ligase were purchased from NEB Company of the United States, primers were synthesized by Invitrogen Company of the United States, 2-deoxy- D-3[H]Glucose was purchased from Amersham, USA.

[0016] 1.2 Experimental m...

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Abstract

The invention belongs to the field of gene engineering, discloses an application of human miR-148a to preparation of medicine for improving adipose cell insulin sensitivity, and provides an application of human miR-148a to preparation of medicine for improving adipose tissue insulin sensitivity and / or promoting glucose uptake of adipose tissue under a state of insulin stimulation in the technical scheme. Researches show that overexpression of the human miR-148a in adipose cells can obviously promote glucose uptake of the adipose cells under the state of insulin stimulation, and the human miR-148a can be used for preparing the medicine for improving adipose tissue insulin sensitivity and / or promoting glucose uptake of the adipose tissue under the state of insulin stimulation as well as used for preparing medicine for preparing diabetes mellitus type 2.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to the application of human miR-148a in the preparation of drugs for improving the insulin sensitivity of adipocytes. Background technique [0002] Diabetes is a metabolic disease that seriously endangers human health and quality of life. At present, type 2 diabetes is the main form of diabetes in my country, accounting for more than 90% of the diabetic population, and the onset of the disease is becoming more and more obvious. Scholars' extensive attention has become an important research direction in the medical field. [0003] The pathogenesis of diabetes mainly has two aspects: one is insufficient insulin secretion, and the other is insulin resistance. Obesity is an important risk factor for insulin resistance. Adipocytes are the classic target cells of insulin action, and their impairment of glucose uptake is an important factor in insulin resistance. Therefore, it has become...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P3/10A61P5/50
Inventor 季晨博郭锡熔史春梅张敏陈玲杨蕾庞玲霞张俊
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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