Unlock instant, AI-driven research and patent intelligence for your innovation.

A microcarrier biochip and its application

A microcarrier and chip technology, applied in microcarrier biochips and its application fields, can solve complex chemical synthesis technology and surface chemical modification technology, complex preparation process, fluorescence resonance energy transfer and other problems, and achieve high-sensitivity parallel detection and detection The effect of simple means and large coding capacity

Active Publication Date: 2016-02-24
CAPITALBIO CORP +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, fluorescence resonance energy transfer may occur between the reporter fluorescence and the coded fluorescence
[0006] At present, bar and two-dimensional coding technologies based on microcarriers or microspheres can realize the coding of samples with large storage capacity, but usually require more complicated chemical synthesis technology and surface chemical modification process
(K.D.JandaandA.R.Vaino, Proc.Natl.Acad.Sci.U.S.A.97 (14), 7692-7696 (2000)) due to involving a large number of synthesis and micro-processing techniques, resulting in poor repeatability and difficult to achieve large-scale production
And it is currently impossible to achieve fast and convenient detection and decoding
[0007] Through the above analysis, it can be found that the existing encoding methods for high-throughput parallel detection generally have disadvantages such as high cost, complicated preparation process, and cumbersome decoding and detection steps. Therefore, there is a need to develop a new type of optical encoding technology. It can easily realize large-capacity encoding and fast decoding, and meet the application needs of high-throughput, low-cost, and parallel detection of biological samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A microcarrier biochip and its application
  • A microcarrier biochip and its application
  • A microcarrier biochip and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the preparation of microcarrier of the present invention and its application effect experiment

[0051] 1. Preparation of microcarriers and their surface films.

[0052] A boron-doped silicon wafer with a crystal form of 100 is used to pass a mixed gas of hydrogen and oxygen at 1000°C. By controlling the reaction time, the thickness of the silicon dioxide film on the surface is controlled. Finally, a dicing process was used to prepare microcarriers with a size of 500 μm×500 μm. Microcarriers with film thicknesses of 93.0 nm, 267.2 nm and 360.7 nm were prepared.

[0053] For a specific film thickness, its reaction time can be obtained by the following formula:

[0054] t = X o 2 B + X o B / A

[0055] Where A and B are constants...

Embodiment 2

[0061] Embodiment 2, microcarrier film thickness resolution verification of the present invention

[0062] Microcarriers with silicon oxide films and silicon substrates were prepared according to the method of step 1 of Example 1, wherein the film thicknesses were 83.2nm, 87.7nm, and 93.0nm.

[0063] Use the above-mentioned system for collecting low-coherence images of micro-carriers to collect low-coherence images of micro-carriers, and analyze its surface color in RGB space to realize decoding. 28.0±2.55), (76.6±2.07, 32.0±2.35, 22.0±1.01), (65.2±0.93, 34.2±1.92, 35.0±1.71),

[0064] Such as Figure 4 As shown, the surface color signals of the three microcarriers with a thickness difference of about 5nm can be clearly distinguished in the three channels of R, G, and B, and through T test analysis, it is found that the signals in the three channels have significant sexual difference. (***: p<0.001; **: p<0.01, *: p<0.05, p<0.05 means there is a significant difference).

...

Embodiment 3

[0066] Embodiment 3, microcarrier sensitivity detection of the present invention

[0067] The film thickness that uses the method described in step 1-2 of embodiment 1 to prepare is the microcarrier of 93.0nm, 267.2nm and 360.7nm, detects target solution according to the method for step 3 in embodiment 1, and target solution is 100ul respectively 5 μg / ml Cy3-labeled goat anti-mouse IgG solution was sequentially used in 1xPBS (preparation method: sodium chloride (NaCl): 8g, potassium chloride (KCl): 0.2g, disodium hydrogen phosphate (NaCl) 2HPO 4 ): 1.44g, potassium dihydrogen phosphate (KH 2 PO 4 ): 0.24g, adjust the pH to 7.4, and set the volume to 1L) for 10, 10 2 、10 3 、10 4 、10 5 For the solution obtained after dilution, use a fluorescence microscope to detect the fluorescence signal to obtain the relationship between the concentration of the target molecule and the fluorescence signal, and use the 3σ principle, that is, use the background fluorescence signal value p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a microcarrier chip and its application. The microcarrier chip comprises a substrate (11), and the substrate (11) is provided with a surface structure capable of generating specific color reflection light under the action of a low-coherence light source. The microcarrier chip is concretely composed of the substrate (11), a transparent film (12) covering the substrate (11), a chemical modification layer (13) of the surface of the transparent film, and a molecular probe (14) fixed on the chemical modification layer in a covalent bond mode or a physical adsorption mode. The microcarrier chip is designed through adopting a low-coherence light based color coding technology to realize the highly sensitive and specific detection of a plurality of biologic molecules. The technology overcomes the periodicity disadvantage of a laser coding technology. The color of the surface of a microcarrier can specifically correspond to the thickness of the film within 1mum. The thickness change resolution of the microcarrier film can reach 5nm through utilizing the color coding / decoding analysis technology.

Description

technical field [0001] The invention relates to a microcarrier biochip and its application, in particular to a microcarrier biochip using low-coherent light interference coding and its application. Background technique [0002] In drug development, new drug screening and rapid diagnosis, it is generally required to perform screening tests on a large number of molecules to be tested. The probes used for library screening are generally specific target molecules, such as antigens, antibodies, nucleic acids, and polypeptides. The screening process actually involves thousands of simultaneous reactions, so the ideal goal is to allow a variety of different probes to perform simultaneous micro-mixing screening. In addition, it is necessary to be able to track the different responses during the screening process. According to this requirement, there are currently two different technical approaches. One is to place all the molecules to be tested in separate multi-well plates and mi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/25G01N33/543
Inventor 李强黄国亮王同舟黎新赵松敏
Owner CAPITALBIO CORP