Rapid neuron staining method based on Golgi silver staining method

Abstract

The invention discloses a rapid neuron staining method based on a Golgi silver staining method. The rapid neuron staining method based on the Golgi silver staining method is characterized in that according to mass volume ratio concentration, the aqueous solution with 5 % of potassium dichromate, the aqueous solution with 5 % of mercuric chloride and the aqueous solution with 5 % of potassium chromate are prepared into a Golgi silver staining solution in the volume ratio of 1:1:1, a brain tissue is soaked in the silver staining solution at 37 DEGCfor 36-48 hours, the soaked brain tissue is sliced on a vibrating slicer, tissue sections are arranged on a glass slide coated with gelatin, the tissue sections stay overnight, then conventional ammonia developing is conducted, gradient alcohol dehydration is conducted, the transparency process is conducted by xylene, finally, the tissue sections are sealed by netrual gum, and the tissue sections are observed through a microscope. According to the rapid neuron staining method based on the Golgi silver staining method, staining can be conducted on each encephalic region of the brain, the staining result is clear, details are obvious, and research staff can observe the neuron structure of the brain tissues conveniently, the convenient and fast means is provided for case analysis of nervous system lesions, and the rapid neuron staining method based on the Golgi silver staining method has significant meanings for the field of foundational research of neurology.

Description

technical field [0001] The invention belongs to the technical field of neuropathological tissue staining, in particular to a rapid nerve tissue staining method based on the Golgi silver staining method. Background technique [0002] Existing scientific studies have shown that the brain nervous system has a type of excitatory cells that are responsible for transmitting and processing nerve signals --- spiny neurons, mainly due to the distribution of small protrusions of various shapes on the dendrites of neurons --- Named after dendritic spines. The fine structure of the dendritic tree of neurons regulates the complex synaptic connections between neurons, and thus regulates the transmission of nerve signals. Dendritic spines are important targets of synaptic input on neurons and are directly related to synaptic plasticity. Therefore, the observation of the changes in the structure of dendrites of spiny neurons provides direct evidence for explaining the complex functional b...

AI Technical Summary

Problems solved by technology

However, there are still a series of problems in this method, such as the whole process is time-consuming, the slices are easily broken into pieces, and the slices are easy to fall off during dyeing, etc.

Especially because the dipping time takes 14 days, it greatly affects the experiment process

At present, many research groups have made some improvements to the dyeing method to avoid the above problems, such as replacing the developer, oxidizing agent, etc., but the shortest experiment time is 5 days. problem, the staining effect is not as good as the original method

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