The invention provides a micro-fluidic
chip device for
nematode culture, storage, capture and
imaging analysis, and relates to the technical field of model animal
nematode research methods, the micro-fluidic
chip device comprises a culture
pool, the top of the culture
pool is open, the length and width of the culture
pool are 1-2 cm, and the depth of the culture pool is 0.3-0.8 cm. The
nematode culture method is the same as the conventional nematode culture method, and the conventional
chip internal closed nematode culture method is changed, so that the
culture environment is the same as that of the conventional culture, and the influence of a chip technology on nematode culture is eliminated. Meanwhile, open culture is more flexible in operation, accepts external operation and is high in
plasticity. According to the device, efficient capturing, fixing,
optical imaging analysis and the like of the nematodes cultured in real time as well as recycling and re-culturing can be completed. The
imaging analysis can be used for
neuron structure imaging,
fluorescence labeling imaging, probe molecule imaging,
ion level
dynamic monitoring,
drug stimulation reaction analysis and the like. The design can also be integrated with other nematode operation analysis modules to be developed into a multifunctional nematode analysis micro-fluidic chip.