Modeling device and method for preparing multiple cerebral infarction from soft autologous blood microembolus

Abstract

The invention discloses a multiple cerebral infarction modeling device and method prepared from soft autologous blood microembolus, and relates to the field of animal modeling. The method comprises the following steps: injecting autologous blood soft microembolus coated with carrageenan into the brain of a rat through an external carotid artery, establishing a multiple cerebral infarction animal model, detecting cerebral infarction disease related experimental markers, observing pathological changes of brain tissues, neuronal structure changes and generation of spatial behavior dysmnesia, and establishing a multiple cerebral infarction animal model. Comprising the steps of red tetrazole staining, hematoxylin-eosin staining, immunohistochemistry, water maze and Bass maze. The rat model proves that the rat has spatial behavior memory disorder, neurons and brain tissues have pathological mechanism changes, and an animal model with similar pathogenesis processes is provided for researching clinical multiple cerebral infarction. The establishment of the animal model has an important value for deepening the treatment research of multiple cerebral infarction diseases.

Description

technical field [0001] The invention relates to the field of animal modeling, in particular to a modeling device and method for preparing multiple cerebral infarction from soft autologous blood microemboli. Background technique [0002] Cerebral infarction is well known for its high mortality, morbidity, and disability rates. Multiple cerebral infarction is another type with a higher proportion. Clinical patients often have functional impairments such as language, behavior, and memory, as well as clinical manifestations such as deviated mouth and eyes, salivation, slurred speech, and inability to stretch and walk normally. Among the general pathological changes, brain tissue softening, necrosis, multiple infarcts, and neuronal loss and structural changes are common. According to research, the risk factors of multiple cerebral infarction are related to many factors including neuroinflammation, cranial aortic atherosclerosis and plaque shedding, and abnormal serum levels of ...

AI Technical Summary

Problems solved by technology

The disadvantage is that the insertion process is easy to cause blood vessel rupture, subarachnoid hemorrhage, etc., resulting in high mortality of experimental animals, and the thread embolization method requires a high level of proficiency in the operation of the experimenter.

Surgical methods include electrocautery of part of the middle cerebral artery and injection of vasoconstrictor through brain stereotaxic craniotomy to cause multiple cerebral infarctions. The surgical method can enter the area closer to the middle cerebral artery, but the disadvantage is that the mortality rate of craniotomy is high, Highly traumatic, and the injected drug dose has a great impact

Embolus injection method, the embolus injection method used today is the method of taking blood and drying it into granules. The embolus produced by this method has the disadvantages of high hardness and high probability of autolysis.

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