Preparation method and application of a novel affinity separation material containing macrocyclic compounds
A technology for macrocyclic compounds and separation materials, applied in the field of preparation of affinity separation materials, can solve problems such as difficult to meet clinical requirements, achieve good mechanical strength and anti-pollution, less time-consuming, and simple operation
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Embodiment 1
[0033] (1) Preparation of blended and separated materials:
[0034] Mix the polymer matrix material EVAL and the solvent DMAc in a certain proportion, add an appropriate amount of β-cyclodextrin, wherein the same content of EVAL is 20%, the solid content of β-cyclodextrin is 2%, and mechanically stir at 40°C for 4h Until completely dissolved, vacuum defoaming, the phase inversion method was used to prepare the separation material, put it in a coagulation bath (deionized water) and let it stand for 3 days, during which the water was changed 5 times, and it was stored in a desiccator for later use.
[0035] (2) Preparation of affinity separation material:
[0036] Take the blended separation material prepared in 3 sheets (1) and put it into 100ml, 1g / L dye solution; the mixed solution is sealed and shaken at 60°C for 30min, then add 20%wt NaCl solution and react for 60min. Heat the water bath to 80°C gradually, add 1%wt Na 2 CO 3 solution to keep the pH value of the mixed sol...
Embodiment 2
[0038] The specific steps for the application of a new type of affinity separation material containing macrocycles using the dye ProcionBlueMX-R as a ligand are as follows:
[0039] (1) Determination of the initial concentration of bovine serum albumin concentration:
[0040] Weigh 0.1 g of each of the 5 groups of affinity separation materials, add buffer and bovine serum albumin at concentrations of 0, 0.2, 0.4, 0.6, and 1.2 mg / ml, and shake at 25°C for 4 hours. The concentration of bovine serum albumin was determined by Coomassie brilliant blue method, and then the adsorption capacity was calculated. figure 1 It is the influence curve of the change of the initial concentration of bovine serum albumin on the adsorption performance. The results show that the adsorption capacity increases with the increase of the initial concentration of bovine serum albumin. Considering comprehensively, the initial concentration of bovine serum albumin is selected as 1.0mg / ml.
[0041] (2) De...
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