Method for adjusting straw fermentation hydrogen production
A straw and anaerobic fermentation technology, which is applied in the field of degrading straw to ferment hydrogen production, can solve the problems of limited lignocellulose structure and complex straw components, and achieve the effect of reducing fermentation cost
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Embodiment 1
[0033] The aldehyde inhibitors 5-HMF and FUR were connected to an atmospheric pressure Erlenmeyer flask hydrogen production test device filled with anaerobic sludge and cultivated for 4.5 days. The atmospheric pressure Erlenmeyer flask hydrogen production test device mainly included a liquid phase sampling port Erlenmeyer flask (total volume 300mL, working volume 150mL), anaerobic bag and airtight plastic tube with three-way sampling port. The hydrogen production test device was placed in an air bath shaker during the experiment, and the culture conditions were a temperature of 37° C. and a rotation speed of 100 rpm. Four groups of conditions were used: Y1 (5-HMF and FUR were not added), Y2 (20mg / L FUR), Y3 (284mg / L 5-HMF), Y4 (284mg / L 5-HMF and 20mg / L FUR). Detect the concentration changes of aldehydes before and after cultivation (as shown in Table 1), and take samples to monitor the daily gas production (such as figure 1 shown).
[0034] As can be seen from Table 1, after...
Embodiment 2
[0042] The aldehyde inhibitor 5-HMF and / or FUR is connected to the test device described in Example 1 containing a mixture of steam-exploded corn stalks and anaerobic sludge, and the culture conditions described in Example 1 are used for anaerobic fermentation to cultivate. First study the comparison of hydrogen production performance under different 5-HMF concentration conditions, monitor the daily hydrogen production and detect the concentration changes of inhibitors before and after cultivation (such as image 3 shown in a). As the concentration of 5-HMF increases from 0mg / L to 2000mg / L, the overall trend of hydrogen production in the system is to increase first and then decrease. After the concentration of 5-HMF exceeds 100mg / L, it begins to decrease, and the hydrogen production is low when the concentration exceeds 500mg / L. In the system without adding 5-HMF. It can be seen from Table 2 that the degradation of 5-HMF corresponds to the generation of hydrogen. Considering t...
Embodiment 3
[0054] Put 200g / L steam-exploded straw (TS content) and anaerobic sludge into a 5L CSTR (fully mixed stirred reactor) hydrogen production reactor, and perform anaerobic fermentation under the culture conditions described in Example 1. Monitor the FUR and 5-HMF content in the reactor every day without adding any exogenous aldehydes fermentation inhibitors (such as Figure 4 shown). Depend on Figure 4 It can be seen that the initial concentration of FUR and 5-HMF is in the range of 50-60mg / L (straw). As the fermentation progresses, both FUR and 5-HMF in the reactor are almost completely degraded within 7 days.
[0055] The control experiment without straw showed that a certain concentration of 5-HMF promoted hydrogen production, while FUR inhibited hydrogen production; the steam-exploded straw system further verified the differential effects of 5-HMF and FUR in the anaerobic fermentation process; In the CSTR hydrogen production reactor system with straw as raw material, almos...
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