Sugarcane tissue culture seedling potassium nutrition research method

Abstract

The invention discloses a sugarcane tissue culture seedling potassium nutrition research method. The method comprises the steps: preparing four culture mediums K1, K2, K3 and K4 with different potassium levels; transplanting tissue culture seedlings with not promoted roots of different sugarcane varieties ROC22, YT55 and YT60 in the culture mediums of test tubes, wherein one seedling is transplanted in one test tube, every variety is finished for 4 times corresponding to K1, K2, K3 and K4, and every finishing is repeated for 6 times; sealing the test tubes by using ventilation sealing films, placing the test tubes wrapped with black films at the culture medium parts in an illumination culture room, setting the temperature as 26 DEG C, the illuminance as 40001x, illumination time is 12 h / day and humidity as 80%, and implementing the culture for 30-45 days; testing the growth index and the potassium content of the cultivated tissue culture seedlings; implementing the data difference significance analysis for the test results. The method can obtain the demands of the tissue culture seedlings of the different sugarcane varieties to the potassium element, so as to provide the basis for cultivating strong sugarcane seedlings.

Description

technical field [0001] The invention relates to the field of seedling nutrition research, in particular to a method for studying potassium nutrition of sugarcane tissue culture seedlings. Background technique [0002] Sugarcane is the most important sugar crop in my country, and its output accounts for more than 85% of the total sugar in the country. Therefore, the cultivation of sugarcane is of great significance to my country's economic development and social stability. Sugarcane is a C4 crop with a large biomass, resulting in a large demand for nutrients, especially for potassium. The study of sugarcane nutrient requirements is particularly important for improving its biomass. [0003] However, the sugarcane growth cycle is long, and the field growth time is about 12 months. Due to the influence of climate, soil and other environmental factors, it is difficult to obtain accurate results in field nutrient tests, and field tests often require a lot of time, manpower and ma...

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Problems solved by technology

[0003] However, the sugarcane growth cycle is long, and the field growth time is about 12 months. Due to the influence of climate, soil and other environmental factors, it is difficult to obtain accurate results in field nutrient tests, and field tests often require a lot of time, manpower and material resources.

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