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A fungus of the genus Congcha and its application in the preparation of fungi-grained wood

A kind of technology of clump genus and fungal pattern wood, applied in the field of microorganisms, can solve the influence of multiple climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, other microbial interference, decay, incomplete wood and other problems , to achieve the effect of being conducive to large-scale production and popularization and application, simple operation process and reasonable technical route

Inactive Publication Date: 2017-04-12
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the naturally obtained fungal wood still has the following deficiencies: 1. The raw materials are not easy to obtain, and there is a strong chance, because the natural fungal wood can only be formed in nature when there are bacteria that can form the fungal wood. , so only a small amount of fungal wood can be found in dead branches and fallen trees in a small number of forests; 2. Natural fungal wood takes a long time to form and is affected by many climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, Other microbial interference, etc.; 3. Natural fungal wood is formed under natural conditions and is susceptible to insect pests or other fungal infections, making the wood incomplete or forming unsightly decay or discoloration

Method used

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  • A fungus of the genus Congcha and its application in the preparation of fungi-grained wood
  • A fungus of the genus Congcha and its application in the preparation of fungi-grained wood
  • A fungus of the genus Congcha and its application in the preparation of fungi-grained wood

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preparation example Construction

[0056] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.

[0057] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.

[0058] The preferred components of the PDA liquid medium in g / L are: potato 200, glucose 15, pH 6.5-7.0.

[0059] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.

[0060] The tree species of the wood-based material in step B can be any tree species.

[0061] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.

[0062] The tree species of the wood-based material in step B is more pref...

Embodiment 1

[0073] - Fungi of the genus Clus Nectria rigidiuscula ) Acquisition, identification and preservation of J12WU.

[0074] (1) Fungus of the genus Congrum ( Nectria rigidiuscula ) Acquisition and identification of J12WU.

[0075] The fungus belonging to the genus Cluschia according to the present invention is isolated from decayed stumps, fallen logs or dead branches of Southwest alder. The samples were collected from the Yunshen Yunshen Scenic Spot in Jindian Park, Kunming City, Yunnan Province. 100g samples of decayed tree stumps and branches with red patterns in the xylem were collected, together with the fungal fruiting bodies growing on them, and stored at 4°C for later use.

[0076] Cut the collected decayed tree stump samples into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for 5 minutes , and then washed 3 times with sterile distilled water. Inoculated in PDA medium, 26...

Embodiment 2

[0088] ——by the fungus of the genus Cluschia ( Nectria rigidiuscula ) J12WU to prepare fungus grain wood.

[0089] (1) Preparation of fungal grain wood.

[0090] First, fungi of the genus Congrum ( Nectria rigidiuscula ) J12WU was inoculated on PDA liquid medium, cultured on a dark shaker at 26°C±2°C for 9 days, and a red mycelium and its mycelium spore suspension were formed.

[0091] The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 15, pH 6.5~7.0.

[0092] Then, the Southwest Alder logs to be inoculated were sawed into square pieces of 2cm×2cm×2cm (12 pieces in total), which were respectively packed in culture bottles with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, cover the bottle cap, place it in a high-pressure steam sterilizer, and sterilize at 121° C. for 40 minutes.

[0093] Clamp...

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Abstract

The invention discloses Nectria rigidiuscula J12WU and application thereof in preparation of bacterium grain wood, belonging to the technical field of microorganisms. The strain is collected in China General Microbiological Culture Collection Center; the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City; and the collection number is CGMCC No:5963. The strain has excellent impregnation capability for a wood-based material, and the impregnation time is short; beautiful colors and grains can be formed on the surface of the material; and the physical mechanical strength is not influenced. A method for preparing a bacterium grain wood by using the strain comprises the steps of strain activation, expanding propagation and inoculation culture; and the method comprises the following specific steps: inoculating the strain on an activation and expanding propagation culture medium for expanding propagation, performing activation and expanding propagation at 22-30 DEG C for 7-15 days, sterilizing the wood-based material, enabling the wood-based material to be sufficiently in contact with the strain subjected to activation and expanding propagation, and culturing at 22-30 DEG C for 1 week or more than 1 week. According to the invention, the method is reasonable in process and simple to operate; and the prepared bacterium grain wood has favorable decoration effect and has no influence on the physical mechanical property of the base material, thereby being beneficial to large-scale production and popularization.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fungus strain of the genus Congchae which has short infection time, beautiful color and texture, and has no influence on the physical and mechanical strength of wood-based materials and its application in the preparation of fungi-patterned wood. Background technique [0002] The fungal discoloration of wood has long been regarded as a defect of wood, which reduces the value of wood. In fact, fungal discoloration, such as lines and staining, has natural and unique characteristics and unique natural beauty. Can be used as a wonderful finishing touch to wood when taken seriously. If this kind of wood with natural lines or colors is used to make handicrafts, such as vases, earrings, pens, wood veneers, etc., the added value of wood can be increased. The fungus wood with natural patterns and colors will be popular in the market, especially in the decoration, decoration...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B27K5/00C12R1/645
Inventor 伍建榕何海珊邱坚罗蓓伍建玲甘昌涛
Owner SOUTHWEST FORESTRY UNIVERSITY
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