A fungus of the genus Congcha and its application in the preparation of fungi-grained wood
A kind of technology of clump genus and fungal pattern wood, applied in the field of microorganisms, can solve the influence of multiple climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, other microbial interference, decay, incomplete wood and other problems , to achieve the effect of being conducive to large-scale production and popularization and application, simple operation process and reasonable technical route
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[0056] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.
[0057] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.
[0058] The preferred components of the PDA liquid medium in g / L are: potato 200, glucose 15, pH 6.5-7.0.
[0059] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.
[0060] The tree species of the wood-based material in step B can be any tree species.
[0061] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.
[0062] The tree species of the wood-based material in step B is more pref...
Embodiment 1
[0073] - Fungi of the genus Clus Nectria rigidiuscula ) Acquisition, identification and preservation of J12WU.
[0074] (1) Fungus of the genus Congrum ( Nectria rigidiuscula ) Acquisition and identification of J12WU.
[0075] The fungus belonging to the genus Cluschia according to the present invention is isolated from decayed stumps, fallen logs or dead branches of Southwest alder. The samples were collected from the Yunshen Yunshen Scenic Spot in Jindian Park, Kunming City, Yunnan Province. 100g samples of decayed tree stumps and branches with red patterns in the xylem were collected, together with the fungal fruiting bodies growing on them, and stored at 4°C for later use.
[0076] Cut the collected decayed tree stump samples into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for 5 minutes , and then washed 3 times with sterile distilled water. Inoculated in PDA medium, 26...
Embodiment 2
[0088] ——by the fungus of the genus Cluschia ( Nectria rigidiuscula ) J12WU to prepare fungus grain wood.
[0089] (1) Preparation of fungal grain wood.
[0090] First, fungi of the genus Congrum ( Nectria rigidiuscula ) J12WU was inoculated on PDA liquid medium, cultured on a dark shaker at 26°C±2°C for 9 days, and a red mycelium and its mycelium spore suspension were formed.
[0091] The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 15, pH 6.5~7.0.
[0092] Then, the Southwest Alder logs to be inoculated were sawed into square pieces of 2cm×2cm×2cm (12 pieces in total), which were respectively packed in culture bottles with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, cover the bottle cap, place it in a high-pressure steam sterilizer, and sterilize at 121° C. for 40 minutes.
[0093] Clamp...
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