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A kind of cotton meal microbial fermentation agent and preparation method thereof

A technology of microbial starter and cottonseed meal is applied in the field of cottonseed meal microbial starter and its preparation, and achieves the effects of reducing acidity, increasing feed remuneration and low preparation cost

Inactive Publication Date: 2016-01-27
SHANGHAI CHUANGBO MODERN NATURAL AGRI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But the lactobacillus starter disclosed in the above-mentioned patent still has some deficiencies in reducing gossypol

Method used

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  • A kind of cotton meal microbial fermentation agent and preparation method thereof
  • A kind of cotton meal microbial fermentation agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of cottonseed meal microbial starter.

[0054] In this example, two strains of Saccharomycescerevisiae with preservation number ACCCNo: 20065 and Lactobacillus plantarum with preservation number ACCCNo: 11118 were selected for breeding and seed production. The steps are as follows:

[0055] Step 1: Cultivate Saccharomyces cerevisiae on potato medium, subculture Lactobacillus plantarum on MRS agar medium, observe the growth rate of the strains, cultivate Saccharomyces cerevisiae colonies with a diameter ≥ 2mm, and plant milk colonies with a diameter ≥ 5mm after culturing at 30°C for 36 hours. The bacterial colony strains were stored at 4°C; the obtained Saccharomyces cerevisiae strains were inoculated in potato medium, and the Lactobacillus plantarum strains were inoculated in MRS medium, and the two strains were shaken at a constant temperature with a rotation speed of 250r / min. Cultivate in the bed at 30°C for 24 hours, take samples, and use the plate count...

Embodiment 2

[0064] Preparation of cottonseed meal microbial starter.

[0065] In this example, two strains of Saccharomycescerevisiae with preservation number ACCCNo: 20065 and Lactobacillus plantarum with preservation number ACCCNo: 11118 were selected for breeding and seed production. The steps are as follows:

[0066] Step 1: Cultivate Saccharomyces cerevisiae on potato medium, subculture Lactobacillus plantarum on MRS agar medium, observe the growth rate of the strains, and cultivate Saccharomyces cerevisiae colonies with a diameter greater than 2mm and plant milk colonies with a diameter greater than 5mm after culturing at 30°C for 40 hours. The bacterial colony strains were stored at 4°C; the obtained Saccharomyces cerevisiae strains were inoculated in potato medium, and the Lactobacillus plantarum strains were inoculated in MRS medium, and the two strains were shaken at a constant temperature with a rotation speed of 250r / min. Cultivate in the bed at 30°C for 24 hours, take samples...

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Abstract

The invention discloses a microbial leavening agent of cottonseed meal as well as a preparation method thereof. The preparation method comprises the following steps: by taking brewer's yeast and lactobacillus plantarum as strains, carrying out enlarging cultivation by means of liquid fermentation; adding a protective agent to prepare the microbial leavening agent, wherein the total viable organisms of the microbial leavening agent is 2*10<9>cfu / g. The microbial leavening agent prepared by the method disclosed by the invention can remarkably degrade free gossypol in cottonseed meal and effectively inhibit growth of infectious microbes in the fermentation process, so that the acidity is reduced and the palatability is improved. In a detoxification fermentation process, a par of plant proteins is further converted into mycoproteins to increase B vitamins and active enzymes so as to have the effect of increasing the protein sources and bio-additives, thereby greatly improving the use value of detoxification fermentation of cottonseed meal. The preparation method is simple in strains and fermentation process, low in cost, good in fermentation effect, low in cottonseed meal fermentation cost and strong in market competitiveness.

Description

technical field [0001] The invention relates to a microbial starter, in particular to a cotton meal microbial starter containing Saccharomyces cerevisiae and Lactobacillus plantarum and a preparation method thereof. Background technique [0002] With the rapid development of aquaculture and feed industry, the relative shortage of protein feed resources has become one of the main factors restricting the development of aquaculture in our country. my country is a big cotton producing country, with an annual output of more than 11 million tons of cottonseed and more than 6 million tons of cottonseed cake. The mass fraction of crude protein in cottonseed meal is 38%-50%, but the digestibility of protein (amino acid) is lower than that of soybean meal, and cottonseed meal contains toxic and harmful substances such as free gossypol, which limits the use of cottonseed meal in feed. application. [0003] At present, the detoxification methods of cottonseed at home and abroad mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18A23K1/14C12R1/25C12R1/865
Inventor 江翰张玉辉
Owner SHANGHAI CHUANGBO MODERN NATURAL AGRI GRP