Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof

A dehairing enzyme and waste technology, which is applied in the field of preparation of immobilized composite dehairing enzymes, can solve the problems of quality of finished leather, poor process controllability, loose surface strength, etc., so as to improve the use performance, improve the controllability, and reduce the degree of effect. Effect

Inactive Publication Date: 2015-07-22
SICHUAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a kind of improved immobilized compound depilatory enzyme and preparation method thereof for the deficiency of existing biological enzyme, to solve the leather quality problem (prone to rotten skin, rotten skin, etc.) rotten surface, loose surface and poor strength accidents), poor process control and other problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof
  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof
  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Soak 100 parts of titanium-containing by-products in deionized water at room temperature for 2 hours, then add 24 parts of glutaraldehyde, shake and react in a water bath at 25°C for 2 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water; weigh 30 parts of compound depilatory enzymes (21 parts of neutral protease, 6 parts of α-amylase, and 3 parts of trypsin) were dissolved in a buffer solution with a pH value of 6.5 and an ionic strength of 0.1 mol / L to prepare a concentration of 6 % compound depilatory enzyme solution, then centrifuged at 5°C and 8000r / min for 10min, and collected the supernatant clear night; the prepared titanium-containing by-products were added to the composite depilatory enzyme solution, so that the ratio of enzyme to titanium-containing by-products was 0.3, Shake and react in a water bath at 5° C. for 1 hour, filter and wash off the residual composite depilatory enzyme on the carrier with deionized water, that...

Embodiment 2

[0032] Soak 100 parts of zirconium-aluminum by-products in deionized water at room temperature for 2 hours, then add 20 parts of glutaraldehyde, shake and react in a water bath at 35°C for 3 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water; Take by weighing 40 parts of compound depilatory enzyme (wherein, 30 parts of neutral protease, 6 parts of α-amylase, 4 parts of trypsin) are dissolved in the buffer solution that pH value is 7.0, and ionic strength is 0.05mol / L, is formulated into Concentration is 6% composite depilatory enzyme solution, centrifuge 10min under 5 ℃, 8000r / min then, collect supernatant; Add the prepared zirconium-aluminum by-products into the composite depilatory enzyme solution, make enzyme and zirconium-containing The proportion of aluminum by-products is 0.4, shaken and reacted in a water bath at 15°C for 0.5h, filtered and washed with deionized water to remove the residual composite depilatory enzyme on the carrie...

Embodiment 3

[0034] Soak 100 parts of zirconium-aluminum-titanium by-products in deionized water at room temperature for 4 hours, then add 15 parts of glutaraldehyde, shake and react in a water bath at 15°C for 3 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water Aldehyde; Take by weighing 20 parts of compound depilatory enzyme (wherein, 16 parts of neutral protease, 2 parts of α-amylase, 2 parts of trypsin) are dissolved in what pH value is 6.5, and ionic strength is in the buffer solution of 0.05mol / L, Prepare a compound hair removal enzyme solution with a concentration of 6%, then centrifuge at 5°C and 8000r / min for 10 minutes, and collect the supernatant; add the prepared zirconium-aluminum-titanium by-products to the compound hair removal enzyme solution to make the enzyme The ratio of zirconium-aluminum-titanium by-products to waste is 0.2, shake and react in a water bath at 25°C for 0.5h, filter and wash off the residual composite depilatory en...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of an immobilized composite unhairing enzyme with leather-making secondary waste as a carrier. The method is characterized by: firstly soaking the leather-making secondary waste in deionized water at room temperature, then reacting the leather-making secondary waste with glutaraldehyde, conducting filtration and washing off the residual glutaraldehyde on the leather-making secondary waste by deionized water; reacting the glutaraldehyde treated carrier with the composite unhairing enzyme, performing filtration and washing off the residual composite enzyme on the carrier, thus obtaining the immobilized composite unhairing enzyme with leather-making secondary waste as the carrier. Specifically, every 100 parts of the composite unhairing enzyme comprise 55-80 parts of neutral protease, 10-25 parts of alpha amylase and 5-15 parts of pancreatin. The immobilized composite unhairing enzyme prepared by the method involved in the invention has a high immobilized enzyme amount and high enzyme activity yield, and has good stability and reusability. The immobilized composite unhairing enzyme can be used for raw hide unhairing and collagen fiber dispersion, the production conditions are mild, high controllability can be achieved on a hide collagen hydrolysis process and the separation and loose degree of collagen fibers, and also the product quality fluctuation can be avoided in production.

Description

technical field [0001] The invention specifically relates to a preparation method of an immobilized compound depilatory enzyme with tanning by-product waste as a carrier, and belongs to the technical field of preparation of immobilized enzymes specially used for tanning. Background technique [0002] As an ancient method, liming and unhairing plays an important role in the tanning process. Conventional ash-alkali hair removal uses sulfide and lime, and through the sulfide and lime to hydrolyze the hair, remove the interstitial fibers, etc. to achieve the effect of hair removal and disperse collagen fibers. Because this method has the advantages of easy access to raw materials, low cost, simple operation, easy control, stable leather quality, and a wide range of applications (all applicable to pigs, cattle, and sheepskin), the current domestic and foreign tanning production is still dominated by ash-alkali depilation. host. However, there are some fatal shortcomings in lime...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C14C1/06
Inventor 胡杨但卫华刘兰但年华
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com