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A method for establishing a rat animal model of irritable bowel syndrome

A technology of irritable bowel syndrome and animal models, which is applied in animal husbandry and other fields, can solve problems such as damage to other body systems, rectal stimulation in rats, etc., and achieve the effect of simple operation, good reproducibility, and improvement of intestinal function

Active Publication Date: 2021-01-19
CHINA INST FOR RADIATION PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the establishment method of the existing animal model of IBS is easy to cause rectal stimulation and damage to other systems of the body in rats.

Method used

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  • A method for establishing a rat animal model of irritable bowel syndrome
  • A method for establishing a rat animal model of irritable bowel syndrome
  • A method for establishing a rat animal model of irritable bowel syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Establishment of Irritable Bowel Syndrome Rat Animal Model (1)

[0019] Twenty-four adult SD male rats, SPF grade, weighing 180-200 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. Before the experiment, they were adaptively raised in the rodent room of the Drug Safety Evaluation Center of the Chinese Academy of Radiation Protection.

[0020] The SD male rats were randomly divided into normal control group and model group according to body weight, with 12 rats in each group. Animals in the normal control group were fed normally. After weighing, the animals in the model group were anesthetized with 10% chloral hydrate (3.5 mL / kg) in a mass-volume ratio, placed on their backs in a fixed box, and treated with Co 60 The radiation source was irradiated to the whole abdomen (the abdomen of the rat was compensated with 1cm tissue, from the xiphoid process to the pubic symphysis, and the rest of the body was shielded with a 5cm ...

Embodiment 2

[0021] Embodiment 2: the establishment of irritable bowel syndrome rat animal model (two)

[0022] Twenty-four adult SD male rats, SPF grade, weighing 180-200 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. Before the experiment, they were adaptively raised in the rodent room of the Drug Safety Evaluation Center of the Chinese Academy of Radiation Protection.

[0023] The SD male rats were randomly divided into normal control group and model group according to body weight, with 12 rats in each group. Animals in the normal control group were fed normally. After weighing, the animals in the model group were anesthetized with 10% chloral hydrate (3.5 mL / kg) in a mass-volume ratio, placed on their backs in a fixed box, and treated with Co 60 The radiation source was irradiated to the whole abdomen (the abdomen of the rat was compensated with 1 cm of tissue, from the xiphoid process to the pubic symphysis, and the rest of the body was shielde...

Embodiment 3

[0024] Embodiment 3: the evaluation of establishment rat animal model

[0025] The rat animal models established in the above-mentioned Example 1 and Example 2 were respectively subjected to the evaluation tests of the following items.

[0026] 1. General conditions of rats

[0027] The mental state, body weight, activity, hair gloss, appetite, and stool properties of the rats were observed.

[0028] Result during the establishment of the rat animal model of embodiment 1 and embodiment 2:

[0029] (1) No rat death occurred;

[0030] (2) Normal control group: the rats were responsive, in good mental state, with good activity, the feces were granular, and the hair was neat and shiny;

[0031] (3) Model group: After the start of modeling, the rats were slightly depressed, their body weight decreased slightly, their activities decreased, they were tired and curled up, their backs were arched, their coats were loose, their coat color was yellowish and less shiny, their abdomens ...

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Abstract

The invention belongs to the technical field of animal model construction, and relates to a method for establishing an animal model of irritable bowel syndrome rats. According to the method, the animal model of irritable bowel syndrome rats is established by combining restraint stress after irradiating the rats with Co60 rays. By adoption of the method for establishing the animal model of irritable bowel syndrome rats, the animal model of irritable bowel syndrome rats can be established with short cycle, low cost, simple operation and good reproducibility, and rectal irritation and other systemic damage to the rats during an animal model establishing process can be reduced or avoided.

Description

technical field [0001] The invention belongs to the technical field of animal model construction and relates to a method for establishing an animal model of irritable bowel syndrome rats. Background technique [0002] Irritable bowel syndrome (irritable bowel syndrome, IBS) is a clinically common and frequently occurring gastrointestinal disease, clinically manifested as abdominal pain or discomfort, abnormal bowel habits and stool properties, etc. But there were no gastrointestinal histomorphological changes and biochemical abnormalities. The prevalence of IBS is about 10-15%. In western countries, IBS accounts for about 20-50% of gastrointestinal outpatient visits. In my country, IBS accounts for about 30-50% of internal medicine outpatient visits and 40-70% of gastroenterology outpatient visits. The prevalence of IBS in women is higher than that in men, and young and middle-aged people are more likely to develop IBS, and the incidence is on the rise. [0003] The etiolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 刘欢高洁黄立群袁慧尹晶晶许文黎岳娟李曙芳孙鸽王新钢李梅秦秀军李建国安全杨彪胡波
Owner CHINA INST FOR RADIATION PROTECTION
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