Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof

A technology of hair removal enzyme and compound enzyme, which is applied in the field of preparation of immobilized compound hair removal enzyme, can solve the problems of finished leather quality, poor process control, loose surface strength, etc., and achieve the goal of improving performance, rich sources, and reducing the degree of action Effect

Inactive Publication Date: 2014-01-22
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide an improved immobilized compound depilatory enzyme and a preparation method thereof for the deficiency of existing biological enzymes, so as to solve the problem of leather quality (prone to rotten skin, rotten skin, etc.) rotten surface, loose surface and poor strength accidents), poor process control and other problems

Method used

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  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof
  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof
  • Immobilized composite unhairing enzyme with leather-making secondary waste as carrier and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Soak 100 parts of titanium-containing by-products in deionized water at room temperature for 2 hours, then add 24 parts of glutaraldehyde, shake and react in a water bath at 25°C for 2 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water; weigh 30 parts of complex enzymes (including 21 parts of neutral protease, 6 parts of α-amylase, and 3 parts of trypsin) were dissolved in a buffer solution with a pH value of 6.5 and an ionic strength of 0.1 mol / L, and the concentration was prepared to be 6%. Then centrifuge at 5°C and 8000r / min for 10min to collect the supernatant; add the prepared titanium-containing by-products to the compound-enzyme solution so that the ratio of enzyme to titanium-containing by-products is 0.3. Shake and react in a water bath for 1 hour, filter and wash away the residual compound enzyme on the carrier with deionized water, that is, the immobilized compound depilatory enzyme with titanium-containing by-products as...

Embodiment 2

[0032] Soak 100 parts of zirconium-aluminum by-products in deionized water at room temperature for 2 hours, then add 20 parts of glutaraldehyde, shake and react in a water bath at 35°C for 3 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water; Weigh 40 parts of the compound enzyme (including 30 parts of neutral protease, 6 parts of α-amylase, and 4 parts of trypsin) and dissolve it in a buffer solution with a pH value of 7.0 and an ionic strength of 0.05 mol / L, and prepare a concentration of 6% compound enzyme solution, then centrifuged at 5°C and 8000r / min for 10min, and collected the supernatant; the prepared zirconium-aluminum by-products were added to the compound enzyme solution to make the enzyme and zirconium-aluminum by-products The ratio is 0.4, shaken and reacted in a water bath at 15°C for 0.5h, filtered and washed with deionized water to remove the residual complex enzyme on the carrier, that is, the immobilized complex depilat...

Embodiment 3

[0034] Soak 100 parts of zirconium-aluminum-titanium by-products in deionized water at room temperature for 4 hours, then add 15 parts of glutaraldehyde, shake and react in a water bath at 15°C for 3 hours, filter and wash off the residual glutaraldehyde on the carrier with deionized water Aldehyde; Weigh 20 parts of compound enzyme (including 16 parts of neutral protease, 2 parts of α-amylase, and 2 parts of trypsin) and dissolve it in a buffer solution with a pH value of 6.5 and an ionic strength of 0.05 mol / L to prepare A compound enzyme solution with a concentration of 6% was prepared, then centrifuged at 5°C and 8000r / min for 10 minutes, and the supernatant was collected; the prepared by-products containing zirconium-aluminum-titanium were added to the enzyme solution to make the enzyme and zirconium-containing The ratio of aluminum-titanium by-products is 0.2, shake and react in a water bath at 25°C for 0.5h, filter and wash off the residual complex enzyme on the carrier ...

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Abstract

The invention discloses a preparation method of an immobilized composite unhairing enzyme with leather-making secondary waste as a carrier. The method is characterized by: firstly soaking the leather-making secondary waste in deionized water at room temperature, then reacting the leather-making secondary waste with glutaraldehyde, conducting filtration and washing off the residual glutaraldehyde on the leather-making secondary waste by deionized water; reacting the glutaraldehyde treated carrier with the composite unhairing enzyme, performing filtration and washing off the residual composite enzyme on the carrier, thus obtaining the immobilized composite unhairing enzyme with leather-making secondary waste as the carrier. Specifically, every 100 parts of the composite unhairing enzyme comprise 55-80 parts of neutral protease, 10-25 parts of alpha amylase and 5-15 parts of pancreatin. The immobilized composite unhairing enzyme prepared by the method involved in the invention has a high immobilized enzyme amount and high enzyme activity yield, and has good stability and reusability. The immobilized composite unhairing enzyme can be used for raw hide unhairing and collagen fiber dispersion, the production conditions are mild, high controllability can be achieved on a hide collagen hydrolysis process and the separation and loose degree of collagen fibers, and also the product quality fluctuation can be avoided in production.

Description

technical field [0001] The invention specifically relates to a preparation method of an immobilized compound depilatory enzyme with tanning by-product waste as a carrier, and belongs to the technical field of preparation of immobilized enzymes specially used for tanning. Background technique [0002] As an ancient method, liming and unhairing plays an important role in the tanning process. Conventional ash-alkali hair removal uses sulfide and lime, and through the sulfide and lime to hydrolyze the hair, remove the interstitial fibers, etc. to achieve the effect of hair removal and disperse collagen fibers. Because this method has the advantages of easy-to-obtain raw materials, low cost, simple operation, easy control, stable leather quality, and a wide range of applications (all applicable to pigs, cattle, and sheepskin), etc., the gray-alkali method is still the main method for tanning production at home and abroad. host. However, there are some fatal shortcomings in lime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C14C1/06
Inventor 胡杨但卫华刘兰但年华
Owner SICHUAN UNIV
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