Extraction method of chanterelle mycelium polysaccharide

An extraction method, a technology of chanterelles, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of high contamination rate of strains, long growth cycle, complex process, etc., and achieve short cultivation cycle, short time, The effect of simple process

Inactive Publication Date: 2014-02-05
甘肃省商业科技研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of chanterelles basically relies on wild resources, and there ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 A method for extracting chanterelle mycelia polysaccharides, comprising the following steps:

[0024] ⑴Preparation of potato glucose slant medium: Wash and peel potatoes, chop them into pieces, add water to boil for 25 minutes according to 4 times its mass, and filter with gauze to obtain filtrate A, make up to 1.0L of filtrate A, add 5g glucose and 5g agar in turn, Make the pH value 6.0~8.0, fully heat and dissolve at 90°C, then sub-package at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0025] ⑵Preparation of seed medium: wash and peel the potatoes, chop them up, add water to boil for 25 minutes according to 4 times its mass, and then filter with gauze to obtain filtrate B. Make up the filtrate B to 1.0L and add 5g of glucose to make the pH value 6.0 ~8.0, after fully heating and dissolving at 90°C, sub-package at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0026] (3) Preparati...

Embodiment 2

[0040] Example 2 A method for extracting chanterelle mycelia polysaccharides, comprising the following steps:

[0041] ⑴Preparation of potato glucose slant medium: Wash and peel the potatoes, chop them up, add water to boil for 35 minutes according to 6 times its mass, and filter with gauze to obtain filtrate A, make up the filtrate A to 1.0L, add 10g glucose and 10g agar in turn, Make it have a pH of 6.0~8.0, fully heat and dissolve at 100°C, and dispense it at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0042] ⑵Preparation of seed medium: Wash and peel the potatoes, chop them up, add water to boil for 35 minutes according to 6 times its mass, and then filter with gauze to obtain filtrate B. Make up the filtrate B to 1.0L and add 10g of glucose to make the pH value 6.0 ~8.0, after fully heating and dissolving at 100°C, sub-package at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0043] (3) Prepar...

Embodiment 3

[0056] Example 3 A method for extracting chanterelle mycelia polysaccharides, comprising the following steps:

[0057] ⑴Preparation of potato glucose slant medium: wash, peel and chop the potatoes, add water to boil for 30 minutes according to 5 times its mass, and filter with gauze to obtain filtrate A, make up to 1.0L of the filtrate A, add 8g glucose and 8g agar in turn, Make the pH value 6.0~8.0, fully heat and dissolve at 95°C, then sub-package at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0058] ⑵Preparation of seed medium: wash and peel the potatoes, chop them up, add water to boil for 30 minutes according to 5 times its mass, and then filter with gauze to obtain filtrate B. Make up the filtrate B to 1.0L and add 8g of glucose to make the pH value 6.0 ~8.0, after fully heating and dissolving at 95°C, sub-package at a pressure of 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C.

[0059] (3) Preparation of fer...

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PUM

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Abstract

The invention relates to an extraction method of chanterelle mycelium polysaccharide. The method comprises the following steps: (1) a potato glucose agarslantculture-medium is prepared; (2) a seed medium is prepared; (3) a fermentation medium is prepared; (4) chanterelle mycelium is inoculated to the potato glucose agarslantculture-medium and undergoes constant temperature incubation to obtain a slant strain; (5) the slant strain of 1cm<2> is inoculated to the seed medium and undergoes shake cultivation to obtain a strain seed liquid; (6) the strain seed liquid is inoculated to the fermentation medium and undergoes shake cultivation to obtain a fermentation broth; and the fermentation broth undergoes centrifugation, washing, drying and grinding to obtain a mycelium dry powder; (7) the mycelium dry powder firstly undergoes supersonic extraction and then is extracted to obtain a polysaccharide leach liquor; (8) the polysaccharide leach liquor undergoes centrifugation, filtering and condensation to obtain a polysaccharide concentrate; (9) a Sevag reagent is added into the polysaccharide concentrate and centrifugation is conducted to obtain a polysaccharide extract; (10) ethanol is added into the polysaccharide extract and standing is conducted to obtain a precipitate; and (11) and the precipitate undergoes centrifugation, washing and drying to obtain the polysaccharide dry material. The extraction method provided by the invention has advantages of simple operation, low cost and high polysaccharide yield.

Description

technical field [0001] The invention relates to the technical field of fungal polysaccharides, in particular to a method for extracting chanterelle mycelia polysaccharides. Background technique [0002] Fungal polysaccharides are a class of macromolecular polymers with extensive pharmacological activities and health care functions. They have various physiological functions such as anti-tumor, anti-infection, anti-coagulation, anti-virus, lowering blood sugar, lowering blood fat, and repairing damaged tissue cells. Common fungal polysaccharides include lentinan, black fungus polysaccharide, Hericium erinaceus polysaccharide, tremella polysaccharide, Ganoderma lucidum polysaccharide, Cordyceps polysaccharide, Yunzhi polysaccharide, Poria polysaccharide, Grifola frondosa polysaccharide and so on. At present, a variety of fungal polysaccharides have been used clinically, which makes the research and development of polysaccharide biological resources increasingly active, and has ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A01G1/04
Inventor 陈朋严晓娟梁宁胡先望
Owner 甘肃省商业科技研究所有限公司
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