Method for marking enveloped virus nucleocapsid by using quantum dot
A technology of quantum dots and nucleocapsids is applied in the field of quantum dots marking enveloped virus nucleocapsids, which can solve the problems of interfering with virus identification virus infection activity, and achieve the effect of maintaining infection activity and a mild method.
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[0023] Example 1 Quantum dot-labeled recombinant baculovirus nucleocapsid
[0024] The method of the present invention will be described in detail below by taking the quantum dot labeling of baculovirus nucleocapsid as an example.
[0025] 1. Method
[0026] 1. Construction of recombinant baculovirus plasmid: using the baculovirus expression system, simultaneously insert the VP39AP sequence (the baculovirus capsid protein VP39 sequence fused with the biotin receptor peptide AP sequence) and the ability to The BirA enzyme sequence that catalyzes AP biotinylation, thereby obtaining a plasmid with the entire genome of the recombinant baculovirus.
[0027] 2. Obtain the purified recombinant baculovirus RBV: transfect the recombinant plasmid into the host Sf9 cells with a transfection reagent, collect the cell supernatant when all the cells have lesions after 72 hours, and pass it at 5000 rpm for 15 min at 4°C Centrifuge to obtain the first generation of recombinant baculovirus...
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