Purpose of CD161 protein

Abstract

The invention provides an antibody specifically bonded with a CD161 protein as well as a purpose of an antibody CD45 / CD3 / CD4 for defining a CD161 expression cell, namely the purpose for preparing a kit for distinguishing latent tuberculosis infection and active tuberculosis. According to the purpose, the expression percentages of the CD161 on a CD3+ cell, a CD3+CD4+ cell and a CD3+CD8+ cell in the blood of tuberculosis patients are obviously lower than the expression percentages in the blood of healthy crowds and latent infection crowds, so that the antibody specifically bonded with the CD161 protein and the antibody CD45 / CD3 / CD4 for defining the CD161 expression cell can be utilized for distinguishing the latent tuberculosis infection and the active tuberculosis, aqnd the tuberculosis diagnosis is more accurate and rapid.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to the use of CD161 protein. Background technique [0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis can not only cause pulmonary tuberculosis (85%), but also cause tuberculosis in multiple organs outside the lungs. Although there are currently effective anti-tuberculosis drugs, tuberculosis is still the number one killer of infectious diseases, and about 2 million people die from tuberculosis every year in the world; about one-third of the world's population is infected with Mycobacterium tuberculosis, which is called About 10% of people with latent tuberculosis infection (LTBI) will eventually develop active tuberculosis. [0003] Due to the lack of effective tuberculosis vaccine at present, the prevention and control of tuberculosis mainly relies on early detection, treatment or isolation o...

AI Technical Summary

Problems solved by technology

However, the current detection technology for diagnosing active tuberculosis cannot meet the clinical and tuberculosis prevention and control requirements, and has the following serious deficiencies: 1) Sputum Mycobacterium tuberculosis microbiological examination is highly specific and is currently the gold standard for diagnosing active tuberculosis, but there are Low sensitivity (less than 40%), long time-consuming (1-2 months for tuberculosis culture), and high requirements for laboratory biosafety

2) Mycobacterium tuberculosis gene detection, although the purpose of rapid diagnosis (1 day) has been achieved, the sensitivity of genetic detection directly from sputum samples has not been significantly improved, and there are problems of false negative or false positive

3) Antibody detection in immunological testing is considered unsuitable for the diagnosis of tuberculosis by the World Health Organization; cellular immunological testing includes tuberculin skin test (TST) and tuberculosis interferon release test (IGRA), which cannot effectively distinguish patients with active tuberculosis and latent tuberculosis infection, although the latter is significantly more sensitive than other tests in patients with active tuberculosis

Although the function of CD161 is not clear yet, recent studies have shown that CD161 is expressed on Treg cells, and CD161+Treg cells express various cytokines such as IFNγ, IL-17, IL-2, and are highly expressed in inflammatory sites

[0005] There is no report on CD161 protein as a diagnostic marker for tuberculosis

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