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Uses of xct inhibitors

A technology for active tuberculosis, applied in the field of bioengineering, to achieve accurate diagnosis of tuberculosis and reduce the effect of bacterial content

Active Publication Date: 2016-01-20
强基广州生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, little is known about the role of xCT in the pathogenesis of infectious diseases, and there is no literature report on the relationship between xCT and tuberculosis

Method used

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  • Uses of xct inhibitors
  • Uses of xct inhibitors
  • Uses of xct inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In this embodiment, the crowd is divided into three groups: tuberculosis patients, latently infected people and healthy people (20 cases each). By detecting the change of xCT gene mRNA in each peripheral blood mononuclear cell (PBMC), it is found that it is present in tuberculosis patients. A clear trend of up-regulated expression.

[0018] In this example, the quantitative RT-PCR method was used to detect the expression changes of xCT gene in each case. Specific steps are as follows:

[0019] Step 1: Preparation of Peripheral Blood Mononuclear Cell (PBMC) Suspension

[0020] Add 5ml of lymphocyte separation medium (FreseniusKabiNOrgeAs: LYS3773) into the centrifuge tube; take 2ml of heparin anticoagulated venous blood from the above-mentioned confirmed tuberculosis patients, patients with latent infection and healthy people, and mix them fully with an equivalent amount of 1M phosphate buffer solution (PBS). Evenly obtain the mixed solution, use a pipette to slowly su...

Embodiment 2

[0049] In this example, a mouse model was used to knock out the xCT gene from the mouse to become an activity gene-deficient mouse. Use Mycobacterium tuberculosis (H37Rv) (from the American Culture Collection Center ATCC) to infect the above-mentioned active gene-deficient mice, and found that the bacterial load in the lungs of the active gene-deficient mice decreased, and the pathology became lighter; at the same time, xCT The gene-specific inhibitor SASP treated mice infected with tuberculosis and found that after taking SASP, it could reduce the bacterial load in the lungs of the mice. The full name of SASP is Sulfasalazine (Sulfasalazine), the product number is S0883, and the CAS number is

[0050] 599-79-1, manufactured by Sigmaaldrich.

[0051] This embodiment specifically includes the following steps:

[0052] Step 1: Infection of mice

[0053] The mice were divided into three groups: Group A, Group B and Group C (6 mice in each group). Among them, group A is xCT ge...

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PUM

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Abstract

The invention provides application of an xCT inhibitor, belongs to the technical field of biological engineering, and particularly relates to application of the xCT inhibitor in preparation of medicines for treating active tuberculosis. The xCT inhibitor is preferably salicylazosulfapyridine. The expression level of the xCT gene in a tuberculosis patient is remarkably higher than that of latent infected and health people, so that the xCT gene can be used as a specific marker gene for diagnosing tuberculosis, so that tuberculosis diagnosis can be accurate and quick; furthermore, tests prove that the specific inhibitor of the xCT gene can be used for remarkably reducing the content of bacteria in a mycobacterium infected mouse body, so that the xCT gene can be used as a new target for treating active tuberculosis.

Description

Technical field: [0001] The invention relates to the technical field of bioengineering, in particular to the use of xCT inhibitors. Background technique: [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (TB) infection. Due to the lack of effective vaccines, the current TB control strategy is mainly early detection and early treatment. Drug treatment is not only the key to reducing the mortality rate of tuberculosis, but also the key to reducing the spread of tuberculosis, so it is the most effective means in the current practice of tuberculosis prevention and control. my country currently uses the short-course chemotherapy regimen recommended by the World Health Organization, including first-line anti-tuberculosis drugs isoniazid (INH), rifampin (RFP), pyrazinamide (PZA), streptomycin (SM), ethambutol (EMB) and other combined treatments. In recent years, quinolones have been increasingly used in the treatment of tuberculosis patien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/635A61P31/06
CPCA61K31/635
Inventor 陈心春蔡毅杨倩婷周伯平
Owner 强基广州生物科技有限公司