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Method for sterile seeding of lithops and establishing regeneration system

A technology for aseptic sowing and lithotripsy, which is applied in the field of plant shoot tip culture and plant tissue culture technology, and can solve problems such as unsuccessful examples.

Active Publication Date: 2014-02-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, before this, there is no report on the establishment of Lithops tissue culture regeneration system, let alone successful examples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present invention provides a method for establishing aseptic seeding and regeneration system of Lithops, the steps of which are:

[0027] 1) Preparation of culture medium

[0028] (1) Basic medium: MS medium, in which sucrose 30g / L, agar 7.5g / L, pH=5.8;

[0029] (2) Induction medium: MS+6-BA0.1~0.5mg / L+NAA0.05~0.1mg / L;

[0030] (3) Differentiation medium: MS+6-BA0~0.05mg / L;

[0031] (4) Proliferation medium: MS+6-BA0.05~0.3mg / L+NAA0.05~0.1mg / L;

[0032] 2) Disinfection and inoculation of seeds

[0033] Take the seeds of Lithops as the explant material, put them in a 1.5ml or 2ml PE centrifuge tube for disinfection, first soak in detergent solution for 0.5h, then wash with tap water, and then in turn the volume ratio is 70 Soak in a% alcohol solution and a sodium hypochlorite solution with an effective chlorine concentration of 1% for 30 seconds and 6 minutes, respectively, and finally rinse with sterile water 3 to 5 times, 2 minutes each time (pipette operation).

[0034] 3) In...

Embodiment 2

[0041] The present invention provides a method for establishing aseptic seeding and regeneration system of Lithops, the steps of which are:

[0042] 1) Preparation of culture medium

[0043] (1) Basic medium: MS medium, in which sucrose 20~30g / L, agar 5~9g / L, pH=5.8;

[0044] (2) Induction medium: MS+6-BA0.2mg / L+NAA0.05mg / L;

[0045] (3) Differentiation medium: MS+6-BA0.05mg / L;

[0046] (4) Proliferation medium: MS+6-BA0.1mg / L+NAA0.05mg / L;

[0047] 2) Disinfection and inoculation of seeds

[0048] Take the seeds of Lithops as the explant material, put them in a 1.5ml or 2ml PE centrifuge tube for disinfection, first soak in detergent solution for 0.5h, then wash with tap water, and then in turn the volume ratio is 70 Soak in a% alcohol solution and a sodium hypochlorite solution with an effective chlorine concentration of 1% for 30 seconds and 6 minutes, respectively, and finally rinse with sterile water 3 to 5 times, 2 minutes each time (pipette operation).

[0049] 3) Induction and diff...

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Abstract

The invention discloses a method for sterile seeding of lithops and establishing a regeneration system. The method specifically comprises the following operation steps: (1) medium preparation, specifically comprising preparation of minimal medium and mediums at various culturing stages; (2) seed disinfection and inoculation; (3) induction and differentiation of a callus; (4) proliferation of adventitious buds; (5) strong seedling culturing. The method has the beneficial effects that the plant tissue culture technology is adopted for carrying out sterile seeding and generated rapid propagation on the lithops, so that a large number of high-quality strong seedlings with consistent inheritable characters are obtained within short periods, the defect of low speed of the conventional propagation method is overcome, and the method has positive significances for large-scale production of seedlings of lithops and collection and storage of germplasm resources.

Description

Technical field [0001] The invention relates to plant tissue culture technology and plant stem tip culture technology, and is mainly a method for aseptic seeding and regeneration system establishment of Lithops. Background technique [0002] Lithops pseudotruncatella (Lithops pseudotruncatella) belongs to the genus Lithops of the Apricot family. Because of its unique shape and colorful colors, it is a very popular small ornamental succulent. Its natural reproduction rate is low, and its place of origin is not in our country, coupled with human over-excavation and destruction of its habitat, the current small quantity and high market price restrict its promotion. Therefore, the use of plant tissue culture technology for the rapid propagation of Afang Gongshou is of great significance for the preservation of excellent germplasm resources and the propagation of famous and rare varieties, so as to achieve large-scale production to meet the needs of domestic and foreign markets. The...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 牟豪杰王燕陈剑平汪一婷吕永平陈志
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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