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Extract of corydalis impatiens total alkaloids and extraction method thereof

A technology for total alkaloids and extracts, which is applied in the field of total alkaloids extracts of the Tibetan medicinal material Sebei Viola and its extraction field, can solve the problems that cannot meet clinical research and application, the product yield is low, and the total alkaloids have medicinal properties. Poor and other problems

Active Publication Date: 2014-03-05
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, the methods for extracting total alkaloids in plants are two kinds of organic solvent extraction and chromatography, and silica gel column chromatography, alumina column chromatography and ion exchange resin are commonly used in chromatography. Although the above methods can all Preparation of total alkaloids, but there are problems of low product yield and high impurity content, and the medicinal properties of the total alkaloids directly obtained by this method are slightly poor, which cannot meet clinical research and application

Method used

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  • Extract of corydalis impatiens total alkaloids and extraction method thereof
  • Extract of corydalis impatiens total alkaloids and extraction method thereof
  • Extract of corydalis impatiens total alkaloids and extraction method thereof

Examples

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Embodiment 1

[0134] Take Corydalis sylvestris medicinal material and crush it into a coarse powder, pass through a 10-mesh sieve, add alkaline ethanol with a volume concentration of 50% saturation, and reflux extraction at 50°C for 3 times, each extraction for 3 hours, to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 10 times the mass of Corydalis serrata medicinal material; the alkaline ethanol is recovered under reduced pressure at 60°C for the above-mentioned Corydalis serrata extract, and then concentrated to a relative density of 1.1 (measured at 50°C). Paste, spray-dried to obtain the alkaline ethanol extract of Corydalis cypresse; adding hydrochloric acid with a volume concentration of 0.5% to the alkaline ethanol extract of Corydalis cypresse to dissolve and filter and repeat 3 times, wherein the amount of solid was added in each dissolution process The acid water liquid of 6 times of amount, merge the filtrate after ...

Embodiment 2

[0136] Take Corydalis sylvestris medicinal material and crush it into a coarse powder, pass through a 50-mesh sieve, add alkaline ethanol with a volume concentration of 95% saturation, and reflux extraction at 70°C for 3 times, each extraction for 2 hours, to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 8 times of the quality of Corydalis serrata medicinal material; the above-mentioned Corydalis serrata extract is recovered under reduced pressure at 60°C for alkaline ethanol, and then concentrated to a relative density of 1.05 (measured at 50°C). paste, spray-dried to obtain the alkaline ethanol extract of Corydalis serrata; adding hydrochloric acid with a volume concentration of 4% in the alkaline ethanol extract of Corydalis serrata to dissolve and filter and repeat 4 times, wherein adding solid mass of 1 times the amount of acid water liquid, the filtrate after merging and filtering forms acid water liquid, a...

Embodiment 3

[0138] Take Corydalis sylvestris medicinal material and crush it into coarse powder, pass through a 50-mesh sieve, add alkaline ethanol with a volume concentration of 90% saturation, and reflux extraction at 80°C for 2.5 hours each time to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 7 times of the quality of Corydalis sylvestris medicinal material; the above-mentioned Corydalis sylvestris extract is recovered under reduced pressure at 60°C for alkaline ethanol, and then concentrated to a relative density of 1.1 (measured at 50°C). Paste, spray-dried to obtain the alkaline ethanol extract of Corydalis sylvestris; adding sulfuric acid with a volume concentration of 1% to the alkaline ethanol extract of Corydalis sylvestris to dissolve and filter and repeat 3 times, wherein the solid amount and mass were added in each dissolution process The sulfuric acid solution of 3 times of amount, merge the filtrate after fil...

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Abstract

The invention discloses an extract of corydalis impatiens total alkaloids, which is extracted by the following steps of grinding the corydalis impatiens medicine, performing reflux extraction by use of saturated alkaline ethanol or saturated alkaline methanol, and performing concentration and drying to obtain a corydalis impatiens extract; dissolving with an acid solution and filtering; adding an alkaline solution into the filtrate, and adjusting the pH value of the solution to 9-10 to obtain acid-alkali treatment liquid; adding an organic solvent into the acid-alkali treatment liquid for extraction to obtain a crude extract of corydalis impatiens total alkaloids; purifying the crude extract to obtain the extract of corydalis impatiens total alkaloids. In the extract of corydalis impatiens total alkaloids obtained by the method disclosed by the invention, the content of total alkaloids is not less than 50wt%, including 10-12wt% of protopine, 8-10wt% of alpha-bicuculline and 10-15wt% of ochotensimine. The study shows that the alkaloid extract has a function of resisting breast cancer, ovarian cancer, stomach cancer and lung cancer.

Description

technical field [0001] The invention relates to an extract of a Chinese herbal medicine and an extracting method thereof, in particular to a total alkaloid extract of a Tibetan medicinal material Corydalis sergei and an extracting method thereof. Background technique [0002] The Tibetan medicine Corydalis Saibei is the dried whole herb of Corydalis saibei (also known as Corydalis saibei), which is a plant of the genus Corydalis Papadaceae. It is distributed in Qinghai, Tibet, Gansu, Sichuan and other places. It is a commonly used drug in Tibetan medicine. The data shows that 25% of Tibetan medicines contain this medicinal material, and the main ingredients in the medicinal material are various alkaloids. In Tibetan medicine, the whole plant of Corydalis genus is often used as medicine, and some of the roots, stems, leaves and other parts of the plant are used as medicine alone. It is mainly used to treat various bleeding symptoms, fever, flu, gastrointestinal and hepatobili...

Claims

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Application Information

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IPC IPC(8): A61K36/66A61P35/00
Inventor 李怀平姬涛魏永义王朔
Owner SHANDONG JINHE DRUG RES DEV
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