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Culture container, culture observation device and culture observation method

A culture container and observation device technology, applied in the field of culture observation, can solve the problems of unsuitability for observation, different cell growth rates, etc.

Active Publication Date: 2017-09-15
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If each well has a different culture environment, the object to be observed, such as the growth rate of cells, etc. is different, which is not suitable for observation

Method used

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  • Culture container, culture observation device and culture observation method
  • Culture container, culture observation device and culture observation method
  • Culture container, culture observation device and culture observation method

Examples

Experimental program
Comparison scheme
Effect test

no. 1 approach ）

[0071] The culture observation device and culture container according to the first embodiment are described.

[0072] [Configuration of culture observation device]

[0073] figure 1 is a schematic diagram of the culture observation device 1 according to the first embodiment. as in figure 1 As shown in , the culture observation device 1 includes an illumination light source 11 , an optical fiber 12 , an illumination optical system 13 , a stage 14 , a microscope optical system 15 , an image capture sensor 16 and a control unit 17 . A culture container 20 in which an object to be observed (cells or the like) is accommodated is installed on the stage 14 . The culture observation device 1 may be a phase contrast microscope that can capture phase contrast images of objects to be observed (cells, etc.).

[0074] The illumination source 11 may be any light source such as a halogen lamp. The illumination light source 11 may be controlled by the control unit 17 in order to control ...

no. 2 approach ）

[0110] [Disposition of culture container]

[0111] A culture container according to a second embodiment will be described. The culture observation device is similar to the first embodiment. Figure 8 is a schematic diagram of a culture container 40 according to the second embodiment.

[0112] Such as Figure 8 As shown, the culture container 40 according to the second embodiment has a side Heater 41. The liquid L is injected into the culture space as in the first embodiment.

[0113] The side heater 41 is a circular heater arranged around the orifice plate 30 when the orifice plate 30 is accommodated in the culture space. The side heater 41 may be a strip heater or a rubber heater. The side heater 41 is controlled by the control unit 17 (see figure 1 ) to a predetermined temperature (eg at 37°C).

[0114] In the first embodiment, the liquid L is injected into the culture space, whereby changes in the culture environment are prevented when the culture container 20 is op...

no. 3 approach ）

[0117] [Disposition of culture container]

[0118] A culture container according to a third embodiment will be described. The culture observation device is similar to the first embodiment. Figure 9 is a schematic diagram of a culture vessel 50 according to the third embodiment.

[0119] Such as Figure 9 As shown, in addition to the frame member 21, the first transparent member 23, the second transparent member 22, the sealing ring 24 and the waterproof member 25 similar to the first embodiment, the culture container 50 according to the third embodiment has a hole plate support member 51 . Unlike the first embodiment, the liquid L is not injected into the culture space.

[0120] The well plate support member 51 is attached to the main body 212 and may support the well plate 30 separated from the first transparent member 23 . The orifice plate support member 51 may be composed of a material having high heat insulating properties.

[0121] The orifice plate supporting mem...

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Abstract

The invention relates to a culture container, a culture observation device and a culture observation method. The culture container includes a first transparent member capable of maintaining a predetermined temperature; a second transparent member facing the first transparent member; a frame member attached to the first transparent member and the second transparent member, and the frame member is connected to the first transparent member A culture space capable of accommodating a well plate is formed together with the second transparent member; and a sealing member is used to seal liquid injected into the culture space between the first transparent member and the frame member.

Description

technical field [0001] The present disclosure relates to a culture container capable of observing an object to be observed being cultured, a culture observation device using the culture container, and a culture observation method. Background technique [0002] When observing an object to be observed such as a living cell, the culture container is used to observe the object to be observed being cultured. The culture vessel is configured to adjust temperature, humidity, gas (e.g. CO 2 ) local pressure, etc., in order to maintain an environment suitable for cultivating the subject to be observed (hereinafter referred to as "cultivation environment"). Generally, a subject to be observed is accommodated in each well of a wellplate (a plate member on which a plurality of wells are arranged), and the subject is kept under a culture environment by housing the wellplate in a culture container. [0003] Here, it is desirable that the culture container can provide the culture environ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34C12Q1/02
CPCC12M23/12C12M23/22C12M37/04C12M41/22C12M41/36C12M23/20C12M25/06C12M29/06C12M31/02C12M41/12C12Q1/02C12M21/02C12M41/14C12Q3/00
Inventor 田中英一芳贺伸介长谷川真皆川达也堂胁优松居惠理子
Owner SONY CORP
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