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A method for improving metal ore leaching rate and its special bacterial strain

A technology for metals and ores, which is applied in the field of improving the leaching rate of metal ores, methods and special strains thereof, can solve the problems of unclear action of Bacillus alicyclic acid, and achieve the advantages of improving leaching rate, good growth and improving leaching efficiency. Effect

Active Publication Date: 2015-09-16
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are some studies on the physiological characteristics and distribution environment of Alicyclobacillus strains (Albuquerque et al., 2000; Chen et al., 2004; Deinhard et al., 1987a&1987b; Goto et al., 2002&2003;) , but there is no report on the use of Alicyclobacillus in the biometallurgical process, and the role of Alicyclobacillus in the bioleaching process is still unclear, so it is necessary to study the role of Alicyclobacillus in the biometallurgical process. role in research

Method used

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  • A method for improving metal ore leaching rate and its special bacterial strain
  • A method for improving metal ore leaching rate and its special bacterial strain
  • A method for improving metal ore leaching rate and its special bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Isolation and Identification of Alicyclobacillus sp. SJ-68 Bacteria

[0050] 1. Separation

[0051] The strain of the present invention is isolated and purified from muddy water samples around sulfur-rich hot springs in Tengchong County, Yunnan Province, China, and the separation and purification time is September 18, 2012.

[0052] The enrichment of microorganisms is carried out by adding 100mL of autotrophic medium into a 250mL shake flask, weighing 5 grams of hot spring mud water sample and adding it into the sterilized autotrophic medium, and enriching and culturing at 30°C. After culturing for one week, the bacterial growth was detected under a microscope. After three times of enrichment, the enrichment medium was diluted 10 times, and 0.2 mL of the diluted medium was spread on a plate to separate single clones. The solid medium was added with 7 g / L Gelrite glue on the basis of the liquid medium. Through multiple streak cultures, a monoclonal strain wa...

Embodiment 2

[0086] Example 2. Application of Alicyclobacillus sp. SJ-68CGMCC No.7682

[0087] 1. Application of SJ-68 combined with Acithiobacillus aldus.SM-1 in dissolving copper sulfide ore

[0088] 1. Preparation of raw materials and inoculation medium

[0089] Yeast extract was purchased from Oxoid Ltd, catalog number 1039501.

[0090] The ore composition of copper sulfide ore: Cu0.8%, Fe2.5%, S3%; % means mass percentage.

[0091] Before the experiment, pass the copper sulfide ore through a 300-mesh sieve (the average particle size is 48 μm), and then use 1:1 (volume ratio of acid and water) H 2 SO 4 The aqueous solution is acidified until the pH value of the obtained acidified solution is maintained between 1.8-2.0 for 24 hours; it takes about 3-5 days; and then the acidified copper sulfide ore is sterilized by ultraviolet light.

[0092] The Alicyclobacillus sp. SJ-68CGMCC No.7682 culture solution used for ore leaching inoculation is to cultivate Alicyclobacillus sp. SJ-68CGMCC...

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Abstract

The present invention discloses a method for increasing a metal ore leaching rate, and a special strain thereof. According to the present invention, Alicyclobacillus sp. SJ-68 is provided, the preservation number is CGMCC No.7682, and a bacterial agent for leaching the target metal from metal ore is further provided, and comprises Alicyclobacillus sp. SJ-68 described in the claim 1 and Acidithiobacillus caldus SM-1 described in the claim 1; and experiment results prove that the Alicyclobacillus sp. SJ-68CGMCC No.7682 can be separately used for leaching the valuable metal in the sulphide ore or be synergistically used with other strains so as to co-leach the valuable metal in the sulphide ore, can be used for deepening extraction of noble metals or rare metals in sulphide ore concentrates, abandoned mine, lean ore, metallurgical slag and difficultly-treated complex sulphide ores, and has important industrial application prospects in the biological mineral leaching field.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the leaching rate of metal ore and a special bacterial strain thereof. Background technique [0002] Biological hydrometallurgy, also known as microbial leaching, is to use certain microorganisms or their metabolites to oxidize, reduce, dissolve, absorb (adsorb) certain minerals (mainly sulfide minerals) and elements, and dissolve them from ores. The hydrometallurgical process of leaching metals or recovering valuable metals from water or removing harmful metals. It has been developed for decades. Due to the favorable factors such as low cost, no pollution, and simple operation, people pay more and more attention to it. The advanced technology has been promoted, perfected and improved worldwide. [0003] For a long time, most of the research and application have focused on the biometallurgical process on acidophilic chemoautotrophs, but in recent years, it h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C22B3/18C12R1/01
CPCY02P10/20
Inventor 刘双江姜成英彭堂见郭旭张明江
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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