Application of prodiginine (PG) in prevention and control of bombyx morinuclear polyhedrosis virus

A nuclear polyhedron and prodigiosin technology, applied in the application field of prodigiosin in the prevention and control of silkworm nuclear polyhedrosis virus disease, can solve the prevention and control of silkworm nuclear polyhedrosis virus disease. There is no research yet Report and other issues to achieve the effect of accelerating the death of nuclear polyhedrosis virus disease silkworms and controlling the spread

Inactive Publication Date: 2014-04-02
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the inhibitory effect of prodigiosin on the virus Bm

Method used

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  • Application of prodiginine (PG) in prevention and control of bombyx morinuclear polyhedrosis virus
  • Application of prodiginine (PG) in prevention and control of bombyx morinuclear polyhedrosis virus
  • Application of prodiginine (PG) in prevention and control of bombyx morinuclear polyhedrosis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment one . Preparation of prodigiosin

[0020] The bacterium that this embodiment adopts is Serratia marcescens ( Serratia marcescens ), Streptomyces ( Streptomyces sp.), Saccharopolyspora ( Saccharopolyspora sp.), Pseudomonas alternata ( Pseudoalteromonas sp.), Actinomyces madura ( Actinomadura sp.).

[0021] 1. Activation of strains

[0022] The preserved Serratia marcescens was inoculated on LB solid medium (1% peptone, 0.5% yeast extract, 1% NaCl, 2% agar; distilled water; pH 7.0), and incubated at 30°C for 48 h. Transplantation was carried out under the same conditions to activate the strain.

[0023] Inoculate the preserved Streptomyces on agar plates (3% yeast extract, 3% peptone, 0.5% glucose, 0.4% maltose, 0.2% MgSO 4 ·7H 2 O, 2% agar; distilled water; pH 7.0), incubated at 30°C for 48 h. Transplantation was carried out under the same conditions to activate the strain.

[0024] The preserved Saccharopolyspora was inoculated on aga...

Embodiment 2

[0042] Embodiment two . Inhibitory effect test of prodigiosin on BmNPV

[0043] The nuclear polyhedrosis virus in this example is derived from the hemolymph of silkworms infected with nuclear polyhedrosis virus, and the experiment is carried out within 30 days after collection and purification.

[0044] 1. BmNPV preparation, cell culture and related reagent configuration

[0045] BmNPV preparation: using Na 2 CO 3 Treat the solution at 28°C for 0.5 h to lyse the BmNPV polyhedron, centrifuge at 3500 rpm for 20 min to remove the precipitate, and collect the supernatant; then centrifuge the collected liquid at 12000 rpm for 30 min, remove the supernatant, collect the precipitate, and the virus particles exist in the precipitate . Cell line: Bombyx mori ovary cell line (BmNS). Cell culture medium: TC-100 insect cell culture medium (Gibco, USA), supplemented with 10% fetal bovine serum (Gibco, USA) and a mixture of penicillin and streptomycin (100 U / mL), pH 6.2. BmNS cells w...

Embodiment 3

[0050] Embodiment Three . Prevention and control effect of prodigiosin on nuclear polyhedrosis virus disease of silkworm

[0051] 1. Toxicity of prodigiosin to individual silkworms

[0052] Mix prodigiosin DMSO solution and distilled water to prepare different doses, spray mulberry leaves to feed silkworms when silkworms emerge from the fifth instar, once a day until they go to the bundle, and count the survival rate of silkworms. The results showed that when prodigiosin was not higher than 10 mg / monkey, it did not show significant toxicity to silkworms. When the concentration of prodigiosin was 10 mg / mox, the intestinal tract and some tissues of silkworms were red, but it did not affect the normal feeding and development of silkworms. Similar results were obtained with the aforementioned five kinds of prodigiosin.

[0053] 2. Prodigiosin for the prevention and control of nuclear polyhedrosis virus disease silkworms

[0054] The silkworms were inoculated with high concent...

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Abstract

The invention relates to the effect of small molecule compound prodiginine (PG) for inhibiting BmNPV (bombyx mori nuclear polyhydrosis virus), and application thereof in prevention and control of BmNPV. Cell assay indicates that PG can interfere with proliferation of BmNPV in cells in a concentration range of 0.01-0.8 mu g/L to stop formation of virus polyhedron, and can selectively cause death of silkworm cells infected with BmNPV in a concentration range of 1-300 mu g/L with the death rate being over 97 percent; PG does not show toxicity to normal cells of silkworm in the application ranges. Individual tests indicate that the silkworms infected with BmNPV stop taking food and prematurely die, infected silkworms can be removed easily, and prodiginine does not show toxicity to healthy cells when prodiginine is used for orally feeding silkworm in a concentration range of 1-10mg per silkworm. Proliferation of BmNPV and formation of polynedron can be inhibited by utilizing PG, and PG has the effect of accelerating death of BmNPV, and can prevent and effectively control spread of BmNPV.

Description

technical field [0001] The invention relates to the field of veterinary drugs, in particular to the application of prodigiosin in preventing and controlling the nuclear polyhedrosis virus disease of silkworm. Background technique [0002] As a traditional aquaculture industry in my country, sericulture is an important source of economic income for farmers in many regions. In 2011, my country's cocoon output was 637,400 tons, with an output value of 21.87 billion yuan, and the silk industry's output value was 203.79 billion yuan. Intensive feeding of silkworms is susceptible to diseases, and the annual loss caused by silkworm diseases to sericulture accounts for about 20% of the total output value of sericulture, of which viral silkworm diseases account for about 80% of the total losses, especially silkworm nuclear polyhedrosis virus ( Bombyx mori nucleopolyhedrovirus, BmNPV) caused the most serious harm of blood type sepsis. Based on the survey of 1782 farmers in 91 counti...

Claims

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Application Information

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IPC IPC(8): A61K31/4025A61K31/4035A61K31/407A61K31/403A61P31/12A23K1/18A23K1/17A23K20/195
Inventor 周围张琳冯丽春敖翔李精华万永继
Owner SOUTHWEST UNIVERSITY
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