Scaffold material capable of blood sugar induced controlled-release of drugs and used for periodontal treatment and preparation method thereof

A scaffold material and controlled-release drug technology, applied in medical science, prostheses, etc., can solve problems such as unsuitable blood sugar-sensing controlled-release drugs, affecting drug protein activity, etc.

Active Publication Date: 2015-06-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing materials fail to form a scaffold structure and suitable pores that are conducive to the growth of periodontal tissue cells, and the preparation method will affect the activity of drug proteins, so it is not suitable for the preparation of scaffold materials for blood sugar-sensitive controlled release drugs for periodontal treatment.

Method used

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  • Scaffold material capable of blood sugar induced controlled-release of drugs and used for periodontal treatment and preparation method thereof
  • Scaffold material capable of blood sugar induced controlled-release of drugs and used for periodontal treatment and preparation method thereof
  • Scaffold material capable of blood sugar induced controlled-release of drugs and used for periodontal treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a scaffold material for blood glucose-sensing controlled-release drugs that can be used for periodontal treatment, specifically implemented according to the following steps:

[0041] 1) preparation of quaternized chitosan;

[0042] At room temperature, chitosan: isopropanol = 1:8 mass ratio is added to the reactor, the pH of the solution is adjusted to 8 with alkali, the water bath is warmed up to 75 ° C, and the concentration of 5 wt% 2,3-cyclo 5 parts of oxypropyltrimethylammonium chloride aqueous solution, constant temperature reaction for 12h, the product was washed with acetone, suction filtered, and freeze-dried to obtain quaternized chitosan;

[0043] 2) Preparation of scaffold materials for blood glucose-sensing controlled release drugs that can be used for periodontal treatment;

[0044] After dissolving collagen in 0.1% acetic acid solution to form a 0.1wt% collagen solution, adjust the pH of the solution to 7 with alkali, according t...

Embodiment 2

[0048] This embodiment provides a scaffold material for blood glucose-sensing controlled-release drugs that can be used for periodontal treatment, specifically implemented according to the following steps:

[0049] 1) Preparation of quaternized chitosan

[0050] At room temperature, chitosan: mass ratio of isopropanol = 1: 15 is added to the reactor, the pH of the solution is adjusted to 13 with alkali, the water bath is warmed up to 99 ° C, and the concentration of 10 wt % 2,3-cyclo 10 parts of oxypropyltrimethylammonium chloride aqueous solution, constant temperature reaction for 24h, the product was washed with acetone, suction filtered, and freeze-dried to obtain quaternized chitosan;

[0051] 2) Preparation of scaffold materials for blood glucose-sensing controlled release drugs that can be used for periodontal treatment;

[0052] Gelatin was used instead of collagen and dissolved in acetic acid to form a 5wt% gelatin solution, and the rest of the process steps and condi...

Embodiment 3

[0055] This embodiment provides a scaffold material for blood glucose-sensing controlled-release drugs that can be used for periodontal treatment, specifically implemented according to the following steps:

[0056] 1) preparation of quaternized chitosan;

[0057] At room temperature, add chitosan: isopropanol = 1: 10 mass ratio in the reactor, adjust the pH of the solution to 10 with alkali, raise the temperature to 80°C in a water bath, and add 2,3-cyclo 8 parts of oxypropyltrimethylammonium chloride aqueous solution, constant temperature reaction for 24h, the product was washed with acetone, suction filtered, and freeze-dried to obtain quaternized chitosan;

[0058] 2) Preparation of scaffold materials for blood glucose-sensing controlled release drugs that can be used for periodontal treatment;

[0059] Sodium alginate protein was used to replace collagen and dissolved in double distilled water to form a 1wt% solution. The rest of the process steps and conditions were the ...

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Abstract

The invention discloses a scaffold material capable of blood sugar induced controlled-release of drugs and used for periodontal treatment and a preparation method thereof. The preparation method is characterized by comprising the following steps: dissolving collagen or gelatin or fibrin in a 0.5 to 5% dilute hydrochloric acid solution or 0.1 to 1% dilute acetic acid solution, or dissolving sodium alginate protein in double distilled water to form a solution with a concentration of 0.1 to 5 wt%; adjusting the pH value of the solution with alkali to 6.5 to 7.5; adding 10 to 20 parts of a natural degradable substance solution, 10 to 20 parts of quaternized chitosan, 1 to 5 parts of glucose oxidase and 5 to 15 parts of anti-inflammatory protein into a reaction vessel and carrying out stirring at a speed of 500 to 5000 rpm at a temperature below 4 DEG C for 10 to 30 min so as to obtain a uniform solution; adding 2 to 6 parts of sodium phosphate into the solution drop by drop; and adding obtained liquid into equipment with a cold eluting agent and into a plastic die with a constant temperature of 4 DEG C, disposing the liquid at a temperature of -20 DEG C for 2 to 4 h, refrigerating the liquid at a temperature of -80 DEG C for 24 to 48 h and carrying out freeze drying at a temperature of -100 DGE C so as to prepare the drug-loaded scaffold material. The scaffold material is stored in equipment with a temperature of 4 DEG C after disinfection by Co60 irradiation.

Description

technical field [0001] The invention relates to a bracket material for periodontal treatment and a preparation method thereof, belonging to the technical field of tissue engineering brackets. Background technique [0002] Periodontitis is one of the important complications of diabetes, which can easily lead to the destruction of periodontal tissue in diabetic patients. Poor glycemic control, i.e. abnormally elevated blood sugar, is common in diabetic patients and can lead to poor periodontal tissue repair after conventional treatment. Obvious periodontal tissue defects often require surgical treatment to receive better curative effect, and poor postoperative blood glucose control will affect the increase of periodontal inflammatory mediators and reduce the healing ability of periodontal tissue. Hyperglycemia promotes an increase in the level of inflammatory mediators such as tumor necrosis factor (TNF-α) in the body, which is an important reason for inhibiting tissue repair...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/54A61L27/22A61L27/24
Inventor 王琪李昊叶林赵晓文肖宇李波邹雪包崇云李伟陈新民
Owner SICHUAN UNIV
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