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Lycoris radiata endophyte with snail killing activity and application thereof

An endophyte and active technology of Lycoris is applied in the field of microorganisms, can solve problems such as unreported, difficult to popularize, and difficult to resource, and achieves the effects of low environmental requirements, conducive to expanding culture, and short growth cycle

Inactive Publication Date: 2014-04-02
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lycoris is rich in a variety of alkaloids, which have high medicinal value and biocontrol effects. Although the use of plants for molluscicide screening and application has been widely studied at home and abroad, the extraction cost of plant molluscicide active ingredients is relatively low. High, resources are also difficult, difficult to promote
Utilize the biological molluscicide obtained by plant endophyte screening, currently only the molluscicidal activity of the endophyte Aspergillus fungus is reported, and it is reported that 10% (volume) of the endophyte Aspergillus fungus The efficiency of killing oncomelania in 48h of the diluted solution is 60%, and the efficiency of killing oncomelania in 72h is 86.7% (Guo Shangbin, Chen Jun, He Jia, etc. Screening of snail-killing active strains of endophytic fungi of Pine pine, Chinese Journal of Schistosomiasis Control, 2007 285-288, 4th Edition, Issue 19, 2019), but there is no report on whether there are strains with molluscicidal activity in Lycoris endophytes

Method used

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  • Lycoris radiata endophyte with snail killing activity and application thereof
  • Lycoris radiata endophyte with snail killing activity and application thereof
  • Lycoris radiata endophyte with snail killing activity and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Screening of Lycoris endophyte CCTCC NO: M2013416 strain:

[0018] 1. Preparation of culture medium:

[0019] 1) YEB liquid medium: beef extract 5.0g / L, yeast extract 1.0g / L, peptone 5.0g / L, MgSO 4 ·7H 2 O is 4g / L, sucrose is 5.0g / L, pH7.0~7.5;

[0020] 2) YEB solid medium: add 15g / L agar to the above YEB liquid medium.

[0021] 2. Screening steps:

[0022] 1) Take Lycoris bulbs, peel off the outer skin and outer bulbs, rinse with tap water for 30 minutes, and soak them in 75% ethanol for 30 seconds under sterile conditions, and then soak them in 0.1% mercury chloride for 15 minutes. Rinse with bacterial water 3 to 5 times to obtain Lycoris tissue;

[0023] 2) Under sterile conditions, mash Lycoris tissue, add 2 mL of sterile saline to obtain tissue slurry;

[0024] 3) Inoculate the tissue slurry from step 2) into 100mL of YEB liquid medium, culture at 25°C for 12-16h, and the rotation speed of the culture is 180rpm to obtain the culture medium;

[0025] 4) Dilut...

Embodiment 2

[0039] This embodiment is the determination of the molluscicidal activity of the endophytic lycoris CCTCC NO: M2013416:

[0040] 1. Preparation of molluscicide:

[0041] Inoculate Lycoris endophyte CCTCC NO: M2013416 in 100mL of YEB liquid medium, culture at 25°C for 24h, and the speed of cultivation is 180rpm to obtain seed liquid, transfer 1mL of seed liquid to 100mL of YEB liquid medium, Cultivate at 25° C. for 12 to 16 hours at a rotational speed of 180 rpm to obtain a fermented liquid, which is a molluscicide.

[0042] 2. Preparation of molluscicide dilution: the molluscicide is prepared with dechlorinated water to make a solution with a volume fraction of 10%, which is the molluscicide dilution.

[0043] 3. Determination of molluscicidal activity:

[0044] After the snails collected in the field were cultured at 25°C for 3 days, the snails with good vigor were selected and rinsed with dechlorinated water three times, and the snails were divided into three groups, with tw...

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Abstract

The invention discloses a lycoris radiata endophyte with snail killing activity, which belongs to Pseudomona sp.N1 and has the biological preservation number of CCTCC (China Center For Type Culture Collection) NO: M2013416. The lycoris radiata endophyte CCTCC NO: M2013416 is screened from the bulbs of a plant lycoris radiata. The snail killing activity of the lycoris radiata endophyte is obviously higher than that of pseudolarix endophytic fungi in the prior part. Meanwhile, compared with the pseudolarix endophytic fungi, the lycoris radiata endophyte CCTCC NO: M2013416 has a shorter growth cycle and has low environment requirement and thus is more favorable for enlargement cultivation. Thus, the invention provides a novel selection for screening a snail-resisting medicine with high efficiency, low toxicity and low cost.

Description

technical field [0001] The invention relates to a microorganism, in particular, the invention relates to an endophytic lycoris with molluscicidal activity and application thereof. Background technique [0002] Schistosomiasis is a parasitic disease that seriously endangers human health. In the middle and lower reaches of the Yangtze River in China, schistosomiasis is prevalent and widely distributed, posing a great threat to people's health. Its only intermediate host is snails, so kill snails It is an effective way to control schistosomiasis. At present, molluscicides are generally used at home and abroad, such as sodium pentachlorophenate, bromoacetamide, nicotinamide and niclosamide, etc. However, the environmental pollution caused by chemical substances used for molluscicide cannot be ignored. Scientific researchers have been committed to finding molluscicides with high molluscicide efficiency, low toxicity to animals and plants, low environmental pollution, and moderat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P9/00C12R1/38
Inventor 张玉琼王梅方陈娜叶爱华张遗
Owner ANHUI AGRICULTURAL UNIVERSITY
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