A kind of actinidin and its preparation method and application
A technology of kiwifruit and kiwifruit root, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of large side effects of chemically synthesized drugs, achieve a good state of mice, promote NK activity, inhibit The effect of worsening development
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Embodiment 1
[0038] Embodiment 1 extracts actinidin
[0039] 1. Preparation of kiwifruit root water extract
[0040] Take 1 kg of decocted kiwi root, add 10L of water, and decoct at 105°C for 3 hours. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.
[0041] 2. Macroporous resin chromatography separation of kiwifruit root water extract
[0042] Take the above-mentioned kiwifruit root water extract, and perform macroporous resin column chromatography. The resin used is D-201, with a column volume of 5L, a column diameter of 8cm, and a flow rate of 1.5 times the column volume / hour. , the elution flow rate was 3 times the column volume / hour, and eluted to colorless; 30% ethanol was eluted, and the elution flow rate was 3 times the column volume / hour, and the elution was colorless.
[0043]Collect the 30% ethanol eluate, concentrate under reduced pressure, vacuum degree -0.8MPa, temperature 50°C, concentrate to obtain an extract with a density o...
Embodiment 2
[0044] Embodiment 2 extracts actinidin with different concentrations of ethanol as eluent
[0045] 1. Preparation of kiwifruit root water extract
[0046] Take 1kg of kiwifruit root, add 9L of water, and decoct in water at 100°C for 3 hours. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.
[0047] 2. Macroporous resin chromatography separation of kiwifruit root water extract
[0048] Take the above-mentioned kiwifruit root water extract, and perform macroporous resin column chromatography, the resin used is D-201, the column volume is 3L, the column diameter is 6cm, and the flow rate is 1 times the column volume / hour. , the elution flow rate is 3 times column volume / hour, elution to colorless; use 10%, 30%, 50%, 70% and 95% ethanol aqueous solution to carry out elution respectively, and described percentage is volume percentage, elution The flow rate was 3 times the column volume / hour, and it was eluted until colorless, and eac...
Embodiment 3
[0049] Embodiment 3 extracts actinidin
[0050] 1. Preparation of kiwifruit root water extract
[0051] Take 1 kg of kiwi fruit root, add 8 L of water, and decoct at 95°C for 1 hour. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.
[0052] 2. Macroporous resin chromatography separation of kiwifruit root water extract
[0053] Take the above-mentioned kiwifruit root aqueous extract, and perform macroporous resin column chromatography, the resin used is D-302, the column volume is 3L, the column diameter is 6cm, the flow rate is 1 times the column volume / hour, and the sample is loaded, and it is eluted with deionized water. The elution flow rate is 3 times the column volume / hour, and the elution is colorless; 30% acetone is eluted, and the elution flow rate is 2 times the column volume / hour, and the elution is colorless.
[0054] Collect 30% acetone eluent, concentrate under reduced pressure, vacuum degree 1.0MPa, temperature 40°...
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