A kind of actinidin and its preparation method and application

A technology of kiwifruit and kiwifruit root, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of large side effects of chemically synthesized drugs, achieve a good state of mice, promote NK activity, inhibit The effect of worsening development

Inactive Publication Date: 2018-03-02
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to provide a kind of actinidin and its preparation method and application in view of the fact that there are few types of drugs for the clinical treatment of malignant tumors and that the chemically synthesized drugs have great side effects.

Method used

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  • A kind of actinidin and its preparation method and application
  • A kind of actinidin and its preparation method and application
  • A kind of actinidin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 extracts actinidin

[0039] 1. Preparation of kiwifruit root water extract

[0040] Take 1 kg of decocted kiwi root, add 10L of water, and decoct at 105°C for 3 hours. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.

[0041] 2. Macroporous resin chromatography separation of kiwifruit root water extract

[0042] Take the above-mentioned kiwifruit root water extract, and perform macroporous resin column chromatography. The resin used is D-201, with a column volume of 5L, a column diameter of 8cm, and a flow rate of 1.5 times the column volume / hour. , the elution flow rate was 3 times the column volume / hour, and eluted to colorless; 30% ethanol was eluted, and the elution flow rate was 3 times the column volume / hour, and the elution was colorless.

[0043]Collect the 30% ethanol eluate, concentrate under reduced pressure, vacuum degree -0.8MPa, temperature 50°C, concentrate to obtain an extract with a density o...

Embodiment 2

[0044] Embodiment 2 extracts actinidin with different concentrations of ethanol as eluent

[0045] 1. Preparation of kiwifruit root water extract

[0046] Take 1kg of kiwifruit root, add 9L of water, and decoct in water at 100°C for 3 hours. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.

[0047] 2. Macroporous resin chromatography separation of kiwifruit root water extract

[0048] Take the above-mentioned kiwifruit root water extract, and perform macroporous resin column chromatography, the resin used is D-201, the column volume is 3L, the column diameter is 6cm, and the flow rate is 1 times the column volume / hour. , the elution flow rate is 3 times column volume / hour, elution to colorless; use 10%, 30%, 50%, 70% and 95% ethanol aqueous solution to carry out elution respectively, and described percentage is volume percentage, elution The flow rate was 3 times the column volume / hour, and it was eluted until colorless, and eac...

Embodiment 3

[0049] Embodiment 3 extracts actinidin

[0050] 1. Preparation of kiwifruit root water extract

[0051] Take 1 kg of kiwi fruit root, add 8 L of water, and decoct at 95°C for 1 hour. The supernatant was collected by centrifugation to obtain the water extract of kiwifruit root.

[0052] 2. Macroporous resin chromatography separation of kiwifruit root water extract

[0053] Take the above-mentioned kiwifruit root aqueous extract, and perform macroporous resin column chromatography, the resin used is D-302, the column volume is 3L, the column diameter is 6cm, the flow rate is 1 times the column volume / hour, and the sample is loaded, and it is eluted with deionized water. The elution flow rate is 3 times the column volume / hour, and the elution is colorless; 30% acetone is eluted, and the elution flow rate is 2 times the column volume / hour, and the elution is colorless.

[0054] Collect 30% acetone eluent, concentrate under reduced pressure, vacuum degree 1.0MPa, temperature 40°...

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Abstract

The invention discloses actinidin, its preparation method and application. The preparation method of the actinidin is as follows: the water extract of the actinidia root is separated by column chromatography with a macroporous resin, the obtained separated product is subjected to alcohol precipitation, and the precipitate is collected. The macroporous resin is a polar macroporous resin, which includes a strongly basic macroporous resin or a weakly basic macroporous resin. Growth and deterioration development; at the same time, it can effectively improve the response function of T lymphocytes, enhance the lethality of mouse splenocytes to target cells, promote NK activity, and improve cellular immune function. When the actinidin inhibits tumor growth, the side effect is very small and has good safety. As a novel low-toxicity antitumor drug, the actinidin may be developed into a new traditional Chinese medicine for treating cancer.

Description

technical field [0001] The invention specifically relates to a kind of actinidin, its preparation method and application. Background technique [0002] Kiwi fruit has a long history of medicinal use. It is recorded in "Materia Medica Supplements" of Tang Dynasty, "Materia Medica Yanyi" of Song Dynasty, and "Compendium of Materia Medica" of Ming Dynasty. It is used to treat hepatitis, edema, rheumatoid arthritis, bruises, dysentery, filariasis, Gastric cancer, breast cancer, etc. Modern pharmaceutical research shows that kiwi fruit has pharmacological activities in reducing blood fat, resisting lipid peroxidation, scavenging active oxygen free radicals, inhibiting tumor cells, and improving immune function. For example, kiwi fruit juice has a significant inhibitory effect on the mutagenic effect of cyclophosphamide (CP)-induced rat peripheral blood binuclear lymphocyte micronucleus cells, and the single-cell gel test of Collins et al. shows that kiwi fruit juice has obvious ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P35/00
Inventor 沈龙海安泳潼尹蓓珮刘畅吴彤刘英
Owner SHANGHAI INST OF PHARMA IND CO LTD
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