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Bacillus mucilaginosus strain and application thereof in alga bio fertilizer

A jelly-like spore and bio-fertilizer technology, applied in the field of microorganisms, can solve the problems of inability to fully extract seaweed nutrients, plant salt damage, etc., achieve strong seaweed degradation ability, fast fertilizer efficiency, and promote high-yield effects

Active Publication Date: 2014-04-09
青岛藻源植物营养有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But in the existing seaweed fertilizer production technology, the digestive agent wherein mostly is based on sodium bicarbonate, sodium hydroxide and inorganic acid, as Chinese invention patent CN98101040, CN03138729 etc., the shortcoming of these technologies is, the process of extracting too much Extensive use of inorganic salts, alkalis or acids will not only fail to fully extract the nutrients in seaweed, but will also cause salt damage to plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) Soaking Ascophyllum nodosum

[0064] Take 50 kg of Ascophyllum nodosum, add 500kg of water according to the ratio of Ascophyllum nodosum: water = 1:10 (weight ratio), soak for 8 hours, filter with a 100-mesh filter, and use the filtrate for later use;

[0065] (2) Physical fragmentation

[0066] Break the Ascophyllum nodosum with a crusher for 30 minutes, and mix it with the soaking water;

[0067] (3) Preparation of fermentation medium

[0068] Take 1000kg of Ascophyllum nodosum mixture obtained in step (2), add 6kg yeast extract powder (0.6%, W / W), 4kg peptone (0.4%, W / W); 1kg sodium chloride (0.1%, W / W) ; 1 kg K 2 HPO 4 (0.1%W / W); 0.1kg MgSO 4 (0.01%, W / W); use ammonia water to adjust the pH of the medium to 7.0;

[0069] (3) Activation of Bacillus jelly-like JK-2 seeds

[0070] The jelly-like Bacillus JK-2 (preservation number is CGMCC No.7549) was transferred from the preserved slant to the fresh test tube slant, and cultured in a constant temperature in...

Embodiment 2

[0076] (1) Kelp soaking

[0077] Take 40 kg of kelp, add 480 kg of water according to the ratio of kelp: water = 1:12 (weight ratio), soak for 15 hours, filter through an 80-mesh filter, and reserve the filtrate;

[0078] (2) Physical fragmentation

[0079] Break the kelp with a crusher for 30 minutes and mix it with soaking water;

[0080] (3) Preparation of fermentation medium

[0081] Take 1000kg of the kelp mixture obtained in step (2), add 8kg of yeast extract powder (0.8%, W / W), 2kg of peptone (0.2%, W / W); 1.2kg of sodium chloride (0.12%, W / W); 0.7kg K 2 HPO 4 (0.07%W / W); 0.3kg MgSO 4 (0.03%, W / W); use ammonia water to adjust the pH of the medium to 7.0;

[0082] (3) Activation of Bacillus jelly-like JK-2 seeds

[0083] The jelly-like Bacillus JK-2 (preservation number is CGMCC No.7549) was transferred from the preserved slant to the fresh test tube slant, and cultured in a constant temperature incubator at 29°C for 24 hours. Take the cultured slant, pick a full ...

Embodiment 3

[0088] (1) Sargassum soaking

[0089] Take 60 kg of sargassum, add 900 kg of water according to the ratio of sargassum: water = 1:15 (weight ratio), soak for 12 hours, filter with a 150-mesh filter, and set aside the filtrate;

[0090] (2) Physical fragmentation

[0091] Break the sargassum with a crusher for 30 minutes, and mix it with the soaking water;

[0092] (3) Preparation of fermentation medium

[0093] Take 1000kg of the sargassum mixture obtained in step (2), add 9kg yeast extract powder (0.9%, W / W), 1kg peptone (0.1%, W / W); 1.5kg sodium chloride (0.1%, W / W) ;0.8kg K 2 HPO 4 (0.08%W / W); 0.4kg MgSO 4 (0.04%, W / W); use ammonia water to adjust the pH of the medium to 7.0;

[0094] (3) Activation of Bacillus jelly-like JK-2 seeds

[0095] The jelly-like Bacillus JK-2 (preservation number: CGMCC No.7549) was transferred from the preserved slant to the fresh test tube slant, and cultured in a constant temperature incubator at 32°C for 24 hours. Take the cultivated ...

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PUM

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Abstract

The invention discloses a bacillus mucilaginosus strain. The bacillus mucilaginosus strain is classified and named as bacillus mucilaginosus, and is preserved in China General Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms (CCCCM) with an accession number of CGMCC No.7549. The invention also relates to application of the bacillus mucilaginosus in preparation of an algae bio fertilizer. The bacillus mucilaginosus strain has strong algae degradation ability, the algae microbial fertilizer prepared from the strain contains rich capsular polysaccharide (or extracellular polysaccharide) produced by breeding and metabolism of the bacillus mucilaginosus and alginic acid, alginate oligosaccharides, gibberellin (GA), indole acetic acid (IAA) and other plant growth regulating active substances produced by biological fermentation degradation conversion of algae compositions, the effective living bacterium number is equal to or greater than 2.0*109CFU / ml, the algae microbial fertilizer is easy to be absorbed directly by crops, is fast in fertilizer efficiency, can effectively promote plant high yield and early maturing, improves the quality of the crops, and is a high quality organic fertilizer special for food products.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a jelly-like bacillus strain and its application in seaweed biological fertilizers. Background technique [0002] Jelly-like Bacillus is Bacillus large capsulata, Gram staining is negative, the capsular polysaccharide is rich, and the spores are oval, which can decompose rock minerals composed of aluminosilicate and silicate, so it is also called silicic acid halobacteria. The microbial fertilizer produced by jelly-like bacillus can be used for foliar spraying and root fertilization of plants, which can promote the absorption of phosphorus and potassium in the soil by plants, reduce the occurrence of plant diseases, increase yield, and improve fruit quality. It is currently widely used An important functional bacteria in the applied microbial fertilizer. [0003] Seaweed (Alga) is algae that grows in the sea and is a cryptogamous plant in the plant kingdom. They are gen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 李园园赵宏涛公春艳王鹏鞠培殿
Owner 青岛藻源植物营养有限公司
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