Bacillus mucilaginosus strain and application thereof in alga bio fertilizer

A jelly-like spore and bio-fertilizer technology, applied in the field of microorganisms, can solve the problems of inability to fully extract seaweed nutrients, plant salt damage, etc., achieve strong seaweed degradation ability, fast fertilizer efficiency, and promote high-yield effects

Active Publication Date: 2014-04-09
青岛藻源植物营养有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But in the existing seaweed fertilizer production technology, the digestive agent wherein mostly is based on sodium bicarbonate, sodium hydroxide and inorganic acid, as Chinese invention patent CN98101040, CN03138729 etc., t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0062] Example 1

[0063] (1) Soaking

[0064] Take 50 kg of Phyllanthus urinaria, add 500kg of water according to the ratio of Phyllanthus vulgaris: water=1:10 (weight ratio), soak for 8 hours, filter with 100 mesh filter, and reserve the filtrate;

[0065] (2) Physically broken

[0066] Use a breaker to break the algae for 30 minutes and mix with the soaking water;

[0067] (3) Preparation of fermentation medium

[0068] Take 1000kg of the Ascophyllum mixed solution obtained in step (2), add 6kg of yeast extract (0.6%, W / W), 4kg of peptone (0.4%, W / W); 1kg of sodium chloride (0.1%, W / W) ; 1kg K 2 HPO 4 (0.1%W / W); 0.1kg MgSO 4 (0.01%, W / W); Adjust the pH of the medium to 7.0 with ammonia water;

[0069] (3) Jelly-like Bacillus JK-2 seed activation

[0070] Transfer the jelly-like Bacillus JK-2 (preservation number CGMCC No. 7549) from the preserved slope to the fresh test tube slope, and incubate it in a constant temperature incubator at 30°C for 24 hours. Take the cultured slope, pick ...

Example Embodiment

[0075] Example 2

[0076] (1) Kelp soaking

[0077] Take 40 kg of kelp, add 480 kg of water according to the ratio of kelp: water = 1:12 (weight ratio), soak for 15 hours, filter with an 80 mesh filter, and reserve the filtrate;

[0078] (2) Physically broken

[0079] Use a breaker to break the kelp for 30 minutes and mix it with the soaking water;

[0080] (3) Preparation of fermentation medium

[0081] Take 1000kg of kelp mixture obtained in step (2), add 8kg of yeast extract (0.8%, W / W), 2kg of peptone (0.2%, W / W); 1.2kg of sodium chloride (0.12%, W / W); 0.7kg K 2 HPO 4 (0.07%W / W); 0.3kg MgSO 4 (0.03%, W / W); Adjust the pH of the medium to 7.0 with ammonia water;

[0082] (3) Jelly-like Bacillus JK-2 seed activation

[0083] Transfer Bacillus jelly-like JK-2 (preservation number CGMCC No. 7549) from the preserved slope to the slope of a fresh test tube, and culture it in a constant temperature incubator at 29°C for 24 hours. Take the cultured slant, pick a full loop with an inoculation ...

Example Embodiment

[0087] Example 3

[0088] (1) Sargassum soaking

[0089] Take 60 kg of sargasso, add 900kg of water at the ratio of sargasso: water = 1:15 (weight ratio), soak for 12 hours, filter with a 150 mesh filter, and use the filtrate for use;

[0090] (2) Physically broken

[0091] Use a breaker to break the Sargassum for 30 minutes and mix with the soaking water;

[0092] (3) Preparation of fermentation medium

[0093] Take 1000kg of Sargassum mixture obtained in step (2), add 9kg of yeast extract (0.9%, W / W), 1kg of peptone (0.1%, W / W); 1.5kg of sodium chloride (0.1%, W / W) ; 0.8kg K 2 HPO 4 (0.08%W / W); 0.4kg MgSO 4 (0.04%, W / W); Adjust the pH of the medium to 7.0 with ammonia water;

[0094] (3) Jelly-like Bacillus JK-2 seed activation

[0095] Transfer the jelly-like Bacillus JK-2 (preservation number CGMCC No. 7549) from the preserved slope to the fresh test tube slope, and incubate in a constant temperature incubator at 32°C for 24 hours. Take the cultured slant, pick a full loop with an in...

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PUM

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Abstract

The invention discloses a bacillus mucilaginosus strain. The bacillus mucilaginosus strain is classified and named as bacillus mucilaginosus, and is preserved in China General Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms (CCCCM) with an accession number of CGMCC No.7549. The invention also relates to application of the bacillus mucilaginosus in preparation of an algae bio fertilizer. The bacillus mucilaginosus strain has strong algae degradation ability, the algae microbial fertilizer prepared from the strain contains rich capsular polysaccharide (or extracellular polysaccharide) produced by breeding and metabolism of the bacillus mucilaginosus and alginic acid, alginate oligosaccharides, gibberellin (GA), indole acetic acid (IAA) and other plant growth regulating active substances produced by biological fermentation degradation conversion of algae compositions, the effective living bacterium number is equal to or greater than 2.0*109CFU / ml, the algae microbial fertilizer is easy to be absorbed directly by crops, is fast in fertilizer efficiency, can effectively promote plant high yield and early maturing, improves the quality of the crops, and is a high quality organic fertilizer special for food products.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a jelly-like bacillus strain and its application in seaweed biological fertilizers. Background technique [0002] Jelly-like Bacillus is Bacillus large capsulata, Gram staining is negative, the capsular polysaccharide is rich, and the spores are oval, which can decompose rock minerals composed of aluminosilicate and silicate, so it is also called silicic acid halobacteria. The microbial fertilizer produced by jelly-like bacillus can be used for foliar spraying and root fertilization of plants, which can promote the absorption of phosphorus and potassium in the soil by plants, reduce the occurrence of plant diseases, increase yield, and improve fruit quality. It is currently widely used An important functional bacteria in the applied microbial fertilizer. [0003] Seaweed (Alga) is algae that grows in the sea and is a cryptogamous plant in the plant kingdom. They are gen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 李园园赵宏涛公春艳王鹏鞠培殿
Owner 青岛藻源植物营养有限公司
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