Straw decomposing agent for quick start of decomposition at low temperature and its preparation method

A straw decomposing agent and quick-start technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, organic fertilizers, etc., can solve the problems of slow decomposing start and incomplete decomposing of decomposing agents, and achieve the effect of improving enzyme activity and decomposing. Good results

Active Publication Date: 2014-04-09
农博士(福建)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of slow start-up and incomplete decomposition of decomposing agents in the prior art, the present invention provides a decomposing agent that can quickly start decomposing at low temperature (below 10°C)

Method used

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  • Straw decomposing agent for quick start of decomposition at low temperature and its preparation method
  • Straw decomposing agent for quick start of decomposition at low temperature and its preparation method

Examples

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Effect test

Embodiment 1

[0023] The decomposing corn stalks were enriched and cultured at low temperature (5°C), and the infusion solution was diluted, and then an appropriate dilution was selected to spread on a double-layer flat plate with cellulose as the only carbon source and cultured at low temperature (5°C) until a single colony was formed. Pick the colonies to obtain the low-temperature decomposing bacteria. It was identified as the Arthrobacter strain YO-H with the preservation number "CGMCC NO.4119".

[0024] Culture the strain YO-H, Bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger, and Trichoderma harzianum separately until the bacterial concentration is 10 8 / mL, mixed evenly according to the volume ratio 1:1:1:2:2, inoculated into 50% wheat bran at a ratio of 1:10 and cultivated for 10 days, and air-dried in a cool place until the water content was ≦25, adding 1%- -1.5% cellulase, mixed evenly to get straw decomposing agent.

[0025] The cultivation method of Arthrobacter ...

Embodiment 2

[0031] The use effect of embodiment 2 decomposing agent of the present invention

[0032] How to use: Organic materials are composted layer by layer. Every layer is 15-20 centimeters, spread evenly layer by layer the decomposing agent of embodiment 1 of the present invention, urea or human and animal excrement and urine, consumption: decomposing agent 2‰; Urea 6-8kg / ton or human and animal excrement and urine 100-200kg / ton, adjust Moisture to 60-70%.

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PUM

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Abstract

The invention relates to a composite microbial inoculant and its preparation method, and overcomes the problems of slow decomposing agent start and incomplete decomposition in the prior art. The invention provides a straw decomposing agent for quick start of decomposition at low temperature, and the straw decomposing agent comprises a strain YO-H, bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger and Trichoderma harzianum. The straw decomposing agent provided by the invention has the advantages that: 1. it employs the strain YO-H, and can quickly start decomposition under the condition of a low environmental temperature; 2. the strain YO-H cooperates with other decomposing fungi to achieve a better decomposing effect; and 3. an additional enzyme preparation is added to improve the cellulose activity of the decomposing agent, about 3-4 days are left before mass propagation of decomposing fungi and generation of new enzyme, and during the period, the additionally added enzyme preparation is employed to degrade lignin and cellulose, that is to say the decomposition time is 3-4 days ahead of schedule.

Description

technical field [0001] The invention relates to a straw decomposing agent and a preparation method thereof, in particular to a straw decomposing agent which rapidly starts decomposing under a low temperature state and a preparation method thereof. Background technique [0002] Organic materials such as straw contain a large amount of lignin, which surrounds hemicellulose and hemicellulose and fills between microfibrils, and is tightly combined with cell wall components. "Prime" constitutes a whole, which increases the difficulty of microbial decomposition of straw. The few days before straw decomposing is the growth and reproduction period of microorganisms in decomposing agent, which contribute little to decomposing. Only when such microorganisms proliferate in large numbers and secrete various lignin and hemicellulose degrading enzymes, destroy the relationship between lignin and hemicellulose. Closely combined, exposing the internal cellulose structure, this enters the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F11/08C12R1/06C12R1/125C12R1/865C12R1/685C12R1/885
Inventor 杜秉海林金新董少英姚良同丁延芹
Owner 农博士(福建)生物技术有限公司
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