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Mumps virus enzyme-linked assay kit

A mumps virus and detection kit technology, applied in the biological field, can solve problems such as uneven quality, high price, and restrictions on widespread use, and achieve high consistency, broad application prospects, and good specificity

Inactive Publication Date: 2014-04-09
WUHAN HANXIONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the quality of various immune detection kits for mumps virus in the domestic market is uneven, there is no uniform quality standard, and the production and application are out of order, causing serious obstacles to the prevention and detection of mumps virus
At present, although foreign reagent manufacturers provide similar commercial detection kits for detection, the price of such kits is very expensive, which limits the widespread clinical use

Method used

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  • Mumps virus enzyme-linked assay kit
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  • Mumps virus enzyme-linked assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Such as figure 1 and figure 2 As shown, a mumps virus enzyme-linked detection kit provided by the present invention includes a box body (15), 8 concave bottle positions (4) built in the box body, 8 bottles of reagent bottle sets, and an enzyme-labeled reaction plate (1) Three sheets of plate sealing film (3) The 8 concave bottle positions are respectively placed with sample diluent (8), enzyme marker (11), concentrated washing solution (12), substrate Solution A (9), Substrate Solution B (7), Stop Solution (5), Negative Control (6), Positive Control (10) each bottle, the enzyme-labeled reaction plate is located behind the reagent bottle, the The sealing film is adjacent to the enzyme-labeled reaction plate; and the size of the sealing film is the same as that of the enzyme-labeled reaction plate; the enzyme-labeled reaction plate includes a support frame (13), and is placed on the support frame There are several detachable micro-well reaction strips (16); 96 micro-re...

Embodiment 2

[0066] Example 2: Using the above kit to detect mumps IgG antibodies in serum by ELISA

[0067] 1. Before the experiment, take the kit out of the refrigerator and leave it at room temperature for 30 minutes before use;

[0068] 2. Dilute the concentrated washing solution 20 times with distilled water before use;

[0069] 3. For each test, set 1 well for positive control and negative control, add 100ul of control solution to each well; 1 blank well (no serum sample diluent, sample and enzyme-labeled antibody, only add substrate A, B and stop solution) ; The rest are sample wells, add serum sample diluent 100ul and sample 1ul to each well. Paste the parafilm and incubate at 37°C for 30 minutes;

[0070] 4. Discard the liquid in the well, pat dry, add 350ul of washing solution to each well, let it stand for 5-10 seconds, discard, pat dry, repeat washing 5 times, and pat dry;

[0071] 5. Add 100ul of enzyme markers, stick a parafilm, and incubate at 37°C for 30 minutes;

[007...

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Abstract

The invention discloses a mumps virus enzyme-linked assay kit. The mumps virus enzyme-linked assay kit comprises a box body, wherein 8 lower concave bottle positions, 8 reagent bottles with sealing covers, a mumps-coated enzyme-labeled reaction plate and three plate sealing films are arranged in the box body, wherein a sample dilution solution, an enzyme label, a concentrated washing solution, a substrate solution A, a substrate solution B, a positive control, a negative control and a stop solution are respectively correspondingly placed in the 8 lower concave bottle positions. The mumps virus enzyme-linked assay kit provided by the invention has high consistency with German EUROIMMUNMedizinischeLabordiagnostikaAG, the sensitivity is 96.67%, the specificity is 96.67%, and the application prospects are further very broad.

Description

technical field [0001] The invention relates to an enzyme-linked detection kit and its application, in particular to designing a mumps virus enzyme-linked detection kit, which belongs to the field of biotechnology. Background technique [0002] There are two types of mumps, bacterial and infectious. This kit detects viral parotitis, commonly known as epidemic parotitis, which is an acute respiratory infectious disease caused by mumps virus invading the parotid gland. It can occur all year round, but it is more common in winter and spring. Most of them occur in young children or teenagers who have a history of infection contact. The incidence rate is higher in children aged 4 to 15, and the symptoms of adults are often more serious than children. It can be bilaterally or unilaterally affected, and the white blood cells not only do not increase but decrease or become normal. The local symptoms are similar to suppurative, but there is no suppurative. After one illness, there ...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/12
Inventor 席甘安军陶杰席昇州
Owner WUHAN HANXIONG BIOTECH INC