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chemically modified graphene

A graphene and protein technology, applied in graphite, inorganic chemistry, organic chemistry, etc., can solve the problems of biomolecular denaturation and separation process, complexity, etc.

Active Publication Date: 2017-03-08
RES FOUND THE CITY UNIV OF NEW YORK +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, such biomolecules may require separation using solvents other than water, which would denature the biomolecules and complicate the separation process

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0093] Embodiment 1: the preparation of the graphene derivative that contains nickel ion

[0094]

[0095] Graphene derivatives containing nickel ions were prepared as follows: the starting material is a suspension containing graphene oxide (GO) with an oxygen content of 8 mol% to 35 mol%, courtesy of Rutgers University Provided by the Nanomaterials and Devices Research Group. All preparations were performed at room temperature. Specifically, a mixture of 10 mM N-hydroxysuccinimide (NHS) and 10 mM N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide (both relative to those on GO 10 mL solution containing 15-20 vol% GO was treated for 1 hour. The suspension was then centrifuged at 21,000×g for 5 minutes, and the precipitate was washed with ultrapure water to obtain a graphene derivative containing an activated carboxyl group. Then with an excess (10 mL) of 150 mM N a ,N a - Treat the washed precipitate with a solution of bis(carboxymethyl)-L-lysine hydrate (pH=9.8) for 1 h...

Embodiment 2

[0097] Example 2: In suspension, the PSII core complex is immobilized on GO-Ni-NTA IMAC resin

[0098] A His-tagged photosystem II core complex (PSII CC) protein on the protein domain CP47 was isolated from the thermophilic cyanobacterium Thermosynechococcus elongatus by standard protocols. The PSII CC protein is a dimeric protein with a physical size of 20.5nm(L)×11.0nm(W)×10.5nm(D) and a molecular weight of 680kDa. The His-tagged PSII CC protein was incubated in suspension containing GO-Ni-NTA resin (prepared in Example 1) for 20 to 60 minutes.

[0099] Coordination of PSII CC proteins to the resin was verified using flow cytometry and the protein loading capacity of the resin was measured. Using an interrogating laser at 488nm, the flow cytometer allows real-time and simultaneous measurement of fluorescence (both edge scatter (SSC) and forward scatter (FSC)) at 692nm. In the presence of a sheath fluid, individual particles undergo hydrodynamic aggregation. SSC and FSC ...

Embodiment 3

[0104] Example 3: PSI core complex protein immobilized on GO-Ni-NTA film

[0105] Functionalization using Ni-NTA groups directly at 1 cm 2 The gold-plated silicon support, which was further coated with GO with an oxygen content of 8-35 wt%, was performed on top of the Si-Au-GO support. The surface treatment process was carried out at room temperature. Specifically, the described Si-Au-GO support for 1 h to activate the carboxyl groups on the GO. After washing the activated Si-Au-GO support three times with ultrapure water, an excess (3 mL) of 150 mM N a ,N a - Bis(carboxymethyl)-L-lysine hydrate (pH=9.8) solution to treat it for 1 hour. The Si-Au-GO support thus obtained was washed three times with ultrapure water to obtain a support containing GO with nitrilotriacetic acid moiety. Then with an excess (3 mL) of 100 mM NiSO 4 Solution processing the washed support results in a support containing GO with nickel ions. In various steps, functionalization was confirmed by...

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Abstract

The present invention relates to graphene derivatives, and related devices comprising graphene derivatives and methods of using graphene derivatives.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. §119, this application claims the following priority: U.S. Provisional Application Serial No. 61 / 488,011 (filed May 19, 2011), U.S. Provisional Application Serial No. 61 / 546,740 (filed October 13, 2011 ), US Provisional Application Serial No. 61 / 601,691 (filed February 22, 2012), and US Provisional Application Serial No. 61 / 601,862 (filed February 22, 2012). The parent application is hereby incorporated by reference in its entirety. [0003] A Note About Federally Sponsored Research [0004] This invention was supported by Contract No. FA9550-10-1-0091 awarded by the Air Force Office of Scientific Research. The US Government has certain rights in this invention. technical field [0005] The present invention relates to graphene derivatives, and related devices and methods. Background technique [0006] Proteins such as enzymes and antibodies are often difficult to isolate while still retaining...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B31/02C01G23/047C07C211/63
CPCG01N33/551C07K1/20B01J20/3085B01J20/3204B01J20/3219B01J20/3236B01J20/3265B01J20/3274B01J20/3289B01J20/205B82Y30/00B82Y40/00B01J45/00B01J39/26B01J2220/58B01D15/325B01D15/3809B01D15/3828B01J20/3251C01B32/23C01B32/194B01J20/223B01J2220/52C07D303/46C25B1/04Y10S977/734Y10S977/847Y10S977/914B01J20/3206C07K1/22C07K2319/20C07K2319/21C12N11/02C12N11/14C12P3/00C12P7/06B01J20/289Y02E50/10Y02E60/36C25B1/55C25B9/40C25B11/051C25B11/057C25B11/095B82Y5/00C07K14/31C07K14/315
Inventor M.维塔德罗K.沃罗诺维茨M.乔瓦拉
Owner RES FOUND THE CITY UNIV OF NEW YORK
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