Healthcare medicinal liquor capable of nourishing and tonifying kidney and preparation method thereof
A health-care medicinal wine and kidney-tonifying technology, which is applied in the preparation of alcoholic beverages, medical formulas, antipyretics, etc., can solve the problems of unfavorable dissolution of fat-soluble components, unfavorable dissolution of water-soluble components, single health care function, etc., and achieve the increase of mouse testis And the weight of epididymis tissue, improving hypoxia resistance and swimming ability, and improving the effect of sperm count
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[0038] Example 1: Preparation of nourishing and nourishing kidney health medicated wine by the preparation method of the present invention
[0039] Weigh 50 grams each of wolfberry, poria cocos, chrysanthemum, polygonatum, cinnamon, yam, and Chinese medicinal materials of Cishi, put into an extraction tank, add 6 liters of 35 degrees high-quality white wine, soak for 2 hours, heat reflux for extraction for 2 hours, filter out the filtrate for later use. Add 3 liters of 35-degree high-quality white wine to the medicine residue for the second hot reflux extraction for 2 hours, filter out the filtrate for use, then add 3.2 liters of 35-degree high-quality white wine to the medicine residue for the third hot reflux extraction for 2 hours, filter out the filtrate, and combine the 3 filtrates , After standing overnight, filter, and the filtrate is divided into aliquots to obtain kidney-tonifying health medicinal wine.
Example Embodiment
[0040] Example 2: Safety evaluation experiment
[0041] Take 1000ml of the medicinal liquor of Example 1 of the present invention and concentrate it under reduced pressure to 50ml, as the medicinal liquor concentrate. Take another 20 clean-grade Kunming mice, weighing 20±2 grams, each male and female, and give the medicinal liquor concentrate once a day in the morning and evening, each time 0.2ml / 10g mouse weight, continuous administration for 7 days, observation The appearance, behavior, appetite, body weight, urine and feces of the mice did not show any abnormalities. None of the 20 mice died. Therefore, the LD50 cannot be calculated. Only the maximum tolerance of oral administration can be roughly estimated. 1:20 concentration> 40ml / kg of body weight, which is more than 1,000 times the clinical dosage, shows that the kidney-tonifying health medicinal wine is safe to eat and has no toxic side effects.
Example Embodiment
[0042] Example 3: Mouse sperm impact test
[0043] Test grouping:
[0044] Commercially available Sanbian wine group (product batch number 20100312), commercially available deer antler wine group (product batch number 20100619), Example 1 group, commercially available Jin wine group (product batch number 20100821), and physiological saline group.
[0045] experiment method:
[0046] Take 50 clean-grade male Kunming mice, weighing 20±2 grams, and randomly divide them into 5 groups. Give the above 4 groups of medicated wine and physiological saline respectively, and re-gavage the rats with 0.1ml / 10g once a day for 7 consecutive days. The animal was sacrificed by removing the cervical spine on 8 days. The epididymis tissue was quickly removed. After weighing with an electronic balance, it was placed in a mortar and ground, diluted 15 times with Ren's solution, and counted the number of sperm under a microscope, and observed its activity. Survival rate, the number of sperm is converted t...
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