Chlamydomonas nivalis immobilizing breed conservation method

Abstract

The invention belongs to the field of microalgae cultivation, and in particular discloses a chlamydomonas nivalis immobilizing breed conservation method. The conventional chlamydomonas nivalis breed conservation method has lots of defects, for example, the breed conservation time is short, particularly 1-2 years; the nutritive salt is rapidly consumed and the culture medium is easy to age and needs to be frequently replaced; therefore, workload is huge, the survival rate of cells is low and cells are likely to be contaminated. According to the method, peptone is used for taking the place of the conventional sodium alginate or agar to be used as a main fixing agent; ferric citrate, potassium sorbate, glucose, sea mud leaching liquor, soil leaching liquor and other nutrients are added to the culture medium ethylene glycol is added as an antifreeze agent, the inoculated culture medium is stored in a low-temperature dim-light incubator with the temperature of -50+ / -2 DEG C and the lighting strength of 200-400lx. By adopting the novel method, the survival rate of the algae cells is remarkably increased, the workload is greatly reduced, the algae cells are hardly contaminated, the germplasm is pure, the breed conservation time can be 6-8 years, and moreover the cost of the breed conservation is reduced to 1 / 3 of that of the original method.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a method for immobilizing and preserving species of polar snow algae. Background technique [0002] Snow algae snow algae is a special group of single-celled microalgae living in polar and similar extreme environments. Dozens of species have been found, belonging to different phyla. Most of them are distributed in the surface layer several centimeters deep in the snow. When the ice and snow melt, snow algal blooms are formed. The cells are rich in astaxanthin and turn dark red, so it is also called blood snow. Among them, the polar snow algae Chlamydomonas nivalis is a freshwater single-cell green algae, which belongs to Chlorophata, Chlorophyceae, and Volvocales in taxonomy. Studies have found that astaxanthin in cells has the effect of anti-ultraviolet radiation. The optimum growth temperature for polar snow algae is 8-10°C. It remains green and grows rapidly...

AI Technical Summary

Problems solved by technology

There are many disadvantages in this method: the seed preservation time is short, 1-2 years, the nutrient salt consumption is fast, the medium is easy to age, the medium needs to be replaced frequently, the workload is large, and the cell survival rate is low, only about 37%. Conservation failure due to pollution