Quantitative detection method for FOC race 4 from soil

Abstract

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

Description

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AI Technical Summary

Benefits of technology

This patented device uses an improved way called Looped Intestinal Amplified Reaction(LIAR), which helps identify bacteria more accurately than previously possible with other techniques like Lampaging or PCR. It also includes features such as fast response time, easy cleanup after use, no contamination from sample extraction, resistance against environmental factors including salts, and stability during storage. Overall this innovative design improves accuracy while reducing costs compared to existing technologies.

Problems solved by technology

This patented describes various techniques for accurately identiting microorganisms such as Bacillium spindle germinates from seeds grown around tomatoes during winter when they occur due to root rotations. These techniques include immunological identification systems like enzymatic resistance technique called Theobromine Acylate Endonuclease(TECHNIS) developed through cloning genome fragments derived from the chromatopathion activaceae species. Other examples involve other biotechnology applications including agricultural crops.

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