Isaria fumosorosea and abamectin oil suspension agent as well as preparation method and application thereof
A technology of Corynebacter rosacea and avermectin oil, which is applied in the field of Corynebacter rosacea and avermectin oil suspension and preparation thereof, and can solve problems such as drug resistance, human harm, environmental pollution and the like , to achieve the effect of strong toxicity, fast lethal speed and environmental safety
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Embodiment 1
[0051] The preparation of embodiment 1 rose fume-colored Isyriamycetes powder
[0052] The I. fumigatus used in the examples of the present invention is I. fumigatus strain SCAU-IFCF02, which was deposited in the China Center for Type Culture Collection on November 1, 2013, with a preservation number of CCTCC NO: M 2013526.
[0053] The preparation process flow chart of the rose fume-colored I. spp. powder of the present invention is as attached figure 1 As shown, the specific method is as follows:
[0054] S1. Take the above-mentioned I. fumigatus strain SCAU-IFCF02, and under sterile conditions, take a ring with an inoculation loop and connect it to SDAY solid medium (a solid spore-forming medium commonly used in fungal culture) for activation. The photoperiod is light:dark=14:10, cultured in a constant temperature incubator at 24°C, and the best culture time is 15 days;
[0055] S2. Pour 10 mL of 0.03% Tween-80 sterile water onto the solid medium described in S1, wash ...
Embodiment 2
[0063] The preparation of embodiment 2 20% I. fumectinus and Abamectin oil suspension
[0064] 1. The preparation process flow chart is attached figure 2 As shown, the specific method is as follows:
[0065] S1. According to the formula requirements, add I. fumoscens powder, vegetable oil, and emulsifier into the mixing kettle, and stir in a high-shear emulsifier machine at 4000r / min for 10 to 15 minutes to fully mix the materials evenly;
[0066] S2. Add the mixed product of S1 to Abamectin original drug, stabilizer, suspending agent, dispersant and mineral oil or methyl oleate, continue high-shear emulsification in the mixing kettle, and stir at 4000r / min for 10 ~15min;
[0067] S3. Put the product obtained in S2 into a sand mill for sand milling to a particle size of 0.5-5 μm to obtain a fume-coloured I. spp. and avermectin oil suspension, which is measured and packaged to obtain a finished product.
[0068] 2. The 20% I. fumectin and avermectin oil suspension prepare...
Embodiment 3
[0069] Example 3 20% Preparation of oil suspension concentrate of I. fumoscens and abamectin
[0070] 1, preparation method: with embodiment 2.
[0071] 2. The 20% I. fumectin and avermectin oil suspension prepared in the present embodiment comprise the following components by weight percentage: I. fumectin powder 18%, abamectin 2%, and 18% soybean oil, 10% alkylphenol polyoxyethylene ether, 2% alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, 3% epoxy linseed oil, 1% organic bentonite, 2% sodium alginate, methyl oleate Ester make up to 100%.
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