Method for detecting viruses and bacteria by fluorescent targeted cells

A technology for targeting cells and fluorescent bacteria, applied in the field of chemical biology, to achieve the effects of high luminous efficiency, good photostability and high fluorescence intensity

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Problems solved by technology

Although great progress has been made in the field of biosynthesis, it is still a great challenge to use living or...

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  • Method for detecting viruses and bacteria by fluorescent targeted cells
  • Method for detecting viruses and bacteria by fluorescent targeted cells
  • Method for detecting viruses and bacteria by fluorescent targeted cells

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Embodiment 1

[0023] The detection of embodiment 1 H9N2 avian influenza virus

[0024] The method of the present invention will be described in detail below by taking the avian influenza H9N2 virus as an example.

[0025] 1. Method

[0026] 1. Construction of fluorescence-targeted bifunctional cells

[0027] Pick a single colony of Staphylococcus aureus from the agarose plate and put into 100 mL of fresh LB medium (yeast extract: 5 g / L, peptone: 10 g / L, sodium chloride: 5 g / L) Incubate at 37°C for 12 h. In this way, 1 mL of bacterial suspension was added to 99 mL of fresh LB medium and continued to incubate at 37 °C for 12 h, then sodium selenite with a final concentration of 5 mM was added to the bacterial suspension, and incubated for 12 h to obtain selenized Staphylococcus aureus. The selenized Staphylococcus aureus was collected by centrifugation, then transferred to 100 mL of fresh LB medium, and then added to the selenized bacterial suspension with a final concentration of...

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Abstract

The invention relates to a method which is capable of efficiently converting a cell into a biological probe and applying the biological probe into the detection for virus and bacteria. The method comprises the steps of successfully applying the original method with 'space-time coupling' new strategy put forward by the laboratory into staphylococcus aureus to enable the interior of the bacteria to be lightened by luminescent quantum dots, and ingeniously establishing a signal report body; due to the natural expression protein A on the surfaces of the cells, carrying out incubation on the fluorescent bacteria and antibody without complex metabolism and genetic engineering cell modification, and carrying out centrifugal washing on excessive antibody to enable the surface of cell wall to be combined with the antibody to obtain the probe with targeting property. The method can be used for capturing the virus or bacteria to be detected by an immune magnetic ball by being combined with an immune magnetic trapping method; and the avian influenza H9N2 virus and the rat cold salmonella are successfully detected by the fluorescent targeted probe. The whole method for establishing the cell probe is simple and ingenious, and the detection process is high in specificity and sensitivity.

Description

technical field [0001] The invention relates to a method for detecting viruses and bacteria by targeting cells with fluorescence, and belongs to the field of chemical biology. Background technique [0002] In recent years, with the development of clean, non-toxic and environmentally friendly "green chemistry", the synthesis of nanomaterials using organic organisms has attracted increasing attention. A large number of organic organisms are used by researchers to synthesize inorganic nanomaterials, such as using Saccharomyces cerevisiae cells as reactors to controllably synthesize fluorescent quantum dots, using Escherichia coli to synthesize CdS nanocrystals, and synthesizing luminescent quantum dots in earthworms. Although great progress has been made in the field of biosynthesis, it is still a great challenge to use living organisms to synthesize high-quality luminescent nanomaterials and further apply intracellular fluorescence to biomedicine. [0003] Our research group ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/64
CPCG01N33/54326G01N33/56911G01N33/56983G01N33/582G01N2333/31
Inventor 庞代文熊玲红崔然
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